Anti-Cofilin 2 抗体 (ab96678)
Key features and details
- Rabbit polyclonal to Cofilin 2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cofilin 2 antibody
Cofilin 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cofilin 2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to a region within amino acids 92 and 152 of Cofilin 2.
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ポジティブ・コントロール
- WB: 293T, A431, HeLaS3, HepG2, MOLT4 or Raji cell lysate; ICC/IF: HeLa cells; IHC-P: gastric carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96678の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 18 kDa.
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IHC-P |
1/100 - 1/250.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 18 kDa. |
IHC-P
1/100 - 1/250. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. -
組織特異性
Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues. -
関連疾患
Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) [MIM:610687]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 7 presents at birth with hypotonia and generalized weakness. Major motor milestones are delayed, but independent ambulation is achieved. -
配列類似性
Belongs to the actin-binding proteins ADF family.
Contains 1 ADF-H domain. -
翻訳後修飾
The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal. -
細胞内局在
Nucleus matrix. Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 1073 Human
- Omim: 601443 Human
- SwissProt: Q9Y281 Human
- Unigene: 180141 Human
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別名
- CFL 2 antibody
- CFL2 antibody
- COF2_HUMAN antibody
see all
画像
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Anti-Cofilin 2 antibody (ab96678) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 18 kDa
12% SDS Page -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96678 at 1/200 dilution.
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Immunohistochemistry analysis of paraffin-embedded gastric carcinoma, using ab96678 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab96678 は 5 報の論文で使用されています。
- Li P et al. Circ_0006404 Accelerates Prostate Cancer Progression Through Regulating miR-1299/CFL2 Signaling. Onco Targets Ther 14:83-95 (2021). PubMed: 33442268
- Wang J et al. Flow-enhanced priming of hESCs through H2B acetylation and chromatin decondensation. Stem Cell Res Ther 10:349 (2019). PubMed: 31775893
- Wo Q et al. Long noncoding RNA SOX2-OT facilitates prostate cancer cell proliferation and migration via miR-369-3p/CFL2 axis. Biochem Biophys Res Commun 520:586-593 (2019). PubMed: 31623830
- Yu BB et al. Cofilin-2 Acts as a Marker for Predicting Radiotherapy Response and Is a Potential Therapeutic Target in Nasopharyngeal Carcinoma. Med Sci Monit 24:2317-2329 (2018). PubMed: 29664897
- Chapnik E et al. miR-142 orchestrates a network of actin cytoskeleton regulators during megakaryopoiesis. Elife 3:e01964 (2014). WB . PubMed: 24859754