Coenzyme A Assay Kit (Fluorometric - Green) (ab138889)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture extracts, Cell Lysate, Tissue Extracts
- Sensitivity: 40 nM
製品の概要
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製品名
Coenzyme A Assay Kit (Fluorometric - Green)
Coenzyme A キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Cell culture extracts, Tissue Extracts, Cell Lysate -
アッセイタイプ
Quantitative -
検出感度
= 40 nM -
全工程の試験時間
1h 00m -
種交差性
交差種: Mammals, Other species -
製品の概要
Coenzyme A Assay Kit (Fluorometric - Green) (ab138889) provides an ultrasensitive fluorometric assay to quantitate CoA content by detection of the –SH group in CoA.
The fluorogenic CoA green indicator dye used in the kit becomes strongly fluorescent upon reacting with –SH.
The assay kit can detect as little as 4 pmol CoA/ 100 µL assay volume (40 nM). It can be performed in a convenient 96-well or 384-well microtiter-plate format at Ex/Em = 490/520 nm, and easily adapted to automation without a separation step.
Coenzyme A assay protocol summary:
- add samples and standards to wells
- add reaction mix
- incubate for 10-60 min whilst measuring fluorescence with a microplate reader
The kit is for solution-based inhibitors or activators of Coenzyme A only. Any samples that contain proteins or thiol-containing compounds are not compatible with the kit.
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特記事項
Previously called Coenzyme A Detection kit (Fluorometric - Green).
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 200 tests CoA Green Indicator 1 vial Assay Buffer 1 x 25ml Coenzyme A Standard 1 x 154µg DMSO 1 x 200µl -
研究分野
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関連性
Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of Coenzyme A is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-Coenzyme A. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates. -
別名
- CoA
- CoASH
- HSCoA
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab138889 は 6 報の論文で使用されています。
- Mignani L et al. Bi-Allelic Mutations in Zebrafish pank2 Gene Lead to Testicular Atrophy and Perturbed Behavior without Signs of Neurodegeneration. Int J Mol Sci 23:N/A (2022). PubMed: 36361705
- Petit F et al. Defective palmitoylation of transferrin receptor triggers iron overload in Friedreich ataxia fibroblasts. Blood 137:2090-2102 (2021). PubMed: 33529321
- Rigby MJ et al. Endoplasmic reticulum acetyltransferases Atase1 and Atase2 differentially regulate reticulophagy, macroautophagy and cellular acetyl-CoA metabolism. Commun Biol 4:454 (2021). PubMed: 33846551
- Kharabsheh HA & Scott JE CoAsy knockdown in TNBC cell lines resulted in no overt effect on cell proliferation in vitro. Biochem Biophys Res Commun 530:136-141 (2020). PubMed: 32828275
- Iuso A et al. A Homozygous Splice Site Mutation in SLC25A42, Encoding the Mitochondrial Transporter of Coenzyme A, Causes Metabolic Crises and Epileptic Encephalopathy. JIMD Rep 44:1-7 (2019). PubMed: 29923093
- Iuso A et al. Mutations in PPCS, Encoding Phosphopantothenoylcysteine Synthetase, Cause Autosomal-Recessive Dilated Cardiomyopathy. Am J Hum Genet 102:1018-1030 (2018). PubMed: 29754768