Application
Western blot
Sample
Human Cell lysate - whole cell (Huh7 cell line)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
20 µg
Specification
Huh7 cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% non-fat milk in TBS-T
Dilution
1/1000
Secondary antibody
Dilution
1/2500
Name
Non-Abcam antibody was used: Dako Polyclonal Goat Anti-Rabbit Immunoglobulins/
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
SuperSignal™ West Femto Maximum Sensitiv
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 65 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 16 2020