保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
バッファーPreservative: 0.09% Sodium azide
Constituents: PBS, 2% Sucrose
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab89720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 141 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
配列類似性Belongs to the neurexin family.
Contains 2 EGF-like domains.
Contains 1 F5/8 type C domain.
Contains 1 fibrinogen C-terminal domain.
Contains 4 laminin G-like domains.
細胞内局在Secreted and Cell membrane.
- Information by UniProt
- CASPR3 antibody
- Cell recognition molecule Caspr3 antibody
- CNTNAP3 antibody
ICC/IF image of ab89720 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab89720 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-CNTNAP3 antibody (ab89720) at 1 µg/ml (in 5% skim milk / PBS buffer) + DU145 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 141 kDa
Observed band size: ~144 kDa why is the actual band size different from the predicted?
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
- Chen Y et al. Angiopoietin-2 (Ang-2) is a useful serum tumor marker for liver cancer in the Chinese population. Clin Chim Acta 478:18-27 (2018). Read more (PubMed: 29253494) »
- Zhao WD et al. Caspr1 is a host receptor for meningitis-causing Escherichia coli. Nat Commun 9:2296 (2018). Read more (PubMed: 29895952) »