CNQX, AMPA / kainate antagonist (ab120017)
Key features and details
- AMPA / kainate antagonist
- CAS Number: 115066-14-3
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
CNQX, AMPA / kainate antagonist -
製品の詳細
AMPA / kainate antagonist -
生理活性の詳細
Potent, competitive AMPA / kainate receptor antagonist. Also antagonist at NMDA receptor glycine site.
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CAS 番号
115066-14-3 -
構造式
製品の特性
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体系名
6-Cyano-7-nitroquinoxaline-2,3-dione -
分子量
232.16 -
分子式
C9H4N4O4 -
PubChem 登録番号
3721046 -
保存方法
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
[O-][N+](=O)c1cc2NC(=O)C(=O)Nc2cc1C#N -
由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120017の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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2D chemical structure image of ab120017, CNQX, AMPA / kainate antagonist
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ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with CNQX (ab120017), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of CNQX, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120017 (CNQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. -
Left and Middle: Representative IOS amplitude map and field response curve under control condition and under application of 20 µM CNQX, respectively. The colorbar indicates the maximum change of the transmittance compared to the resting light intensity. A Right: Spatial visualization of the percentage of control changes of IOS signal caused by CNQX application.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (71)
ab120017 は 71 報の論文で使用されています。
- Mouradian GC et al. Patch-to-Seq and Transcriptomic Analyses Yield Molecular Markers of Functionally Distinct Brainstem Serotonin Neurons. Front Synaptic Neurosci 14:910820 (2022). PubMed: 35844900
- Kleidonas D & Vlachos A Scavenging Tumor Necrosis Factor a Does Not Affect Inhibition of Dentate Granule Cells Following In Vitro Entorhinal Cortex Lesion. Cells 10:N/A (2021). PubMed: 34831454
- Sanders SS et al. The palmitoyl acyltransferase ZDHHC14 controls Kv1-family potassium channel clustering at the axon initial segment. Elife 9:N/A (2020). PubMed: 33185190
- Mangieri LR et al. A neural basis for antagonistic control of feeding and compulsive behaviors. Nat Commun 9:52 (2018). PubMed: 29302029
- Soh H et al. Deletion of KCNQ2/3 potassium channels from PV+ interneurons leads to homeostatic potentiation of excitatory transmission. Elife 7:N/A (2018). PubMed: 30382937