Anti-Clostridium botulinum E Toxoid 抗体 [209F2] (ab40787)
Key features and details
- Mouse monoclonal [209F2] to Clostridium botulinum E Toxoid
- Suitable for: ICC/IF, Inhibition Assay
- Reacts with: Clostridium botulinum
- Isotype: IgG
製品の概要
-
製品名
Anti-Clostridium botulinum E Toxoid antibody [209F2] -
製品の詳細
Mouse monoclonal [209F2] to Clostridium botulinum E Toxoid -
由来種
Mouse -
特異性
BoTox-E (2-17) 100%; BoTox-E 100% Does not cross react with BoTox-A, BoTox-B, BoTox-C or BoTox-F (1-16) -
アプリケーション
適用あり: ICC/IF, Inhibition Assaymore details -
種交差性
交差種: Clostridium botulinum -
免疫原
Synthetic peptide analogue of the amino terminal region of BoTox-E.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Constituent: Tissue culture supernatant -
Concentration information loading...
-
精製度
Tissue culture supernatant -
特記事項(精製)
Sterile filtered culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
209F2 -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab40787の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | ||
Inhibition Assay |
特記事項 |
---|
This product has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of BoTox-E at a dilution of 1:50 using an indirect immunocytochemical staining procedure.
Inhib: Use at an assay dependent dilution.
This product has been found to inhibit the entry of BoTox-E to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin.
With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
-
関連性
Clostridium botulinum E Toxoid acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase. It has a non-toxic component which is necessary to maintain toxicity. -
細胞内局在
Secreted -
参照データベース
- SwissProt: Q00496 Clostridium botulinum
-
別名
- BoNT/E antibody
- Bontoxilysin e antibody
- Botulinum neurotoxin type e non toxic component antibody
see all
データシートおよび資料
-
Datasheet download
参考文献 (1)
ab40787 は 1 報の論文で使用されています。
- Shu Y et al. Phosphorylation of SNAP-25 at Ser187 mediates enhancement of exocytosis by a phorbol ester in INS-1 cells. J Neurosci 28:21-30 (2008). PubMed: 18171919