Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6495] to CLIC2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Rat, Human
製品名Anti-CLIC2 antibody [EPR6495] - BSA and Azide free
CLIC2 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR6495] to CLIC2 - BSA and Azide free
アプリケーション適用あり: WBmore details
適用なし: IHC-P or IP
種交差性交差種: Rat, Human
Synthetic peptide within Human CLIC2 aa 200-300. The exact sequence is proprietary.
Database link: O15247
ab248151 is the carrier-free version of ab126727 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248151 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 3.10 x 10 -11 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).|
機能Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Modulates the activity of RYR2 and inhibits calcium influx.
組織特異性Detected in adult brain, heart, liver, lung, spleen, stomach and testis. Expressed in fetal liver and adult skeletal muscle.
配列類似性Belongs to the chloride channel CLIC family.
Contains 1 GST C-terminal domain.
ドメインMembers of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
細胞内局在Cytoplasm. Membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain.
- Information by UniProt
- Chloride intracellular channel 2 antibody
- Chloride intracellular channel protein 2 antibody
- CLIC 2b antibody
All lanes : Anti-CLIC2 antibody [EPR6495] (ab126727) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human heart lysate
Lane 3 : Human testis lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
This data was developed using ab126727, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.