Anti-Cleaved PARP1 抗体 (ab4830)

Reviews (4)Q&A (3)References (43)
Western blot - Anti-Cleaved PARP1 antibody (ab4830)
  • Western blot - Anti-Cleaved PARP1 antibody (ab4830)
  • Western blot - Anti-Cleaved PARP1 antibody (ab4830)

Key features and details

  • Rabbit polyclonal to Cleaved PARP1
  • Suitable for: WB
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-PARP1 antibody [EPR18461] (ab191217)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Cleaved PARP1 antibody
    Cleaved PARP1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Cleaved PARP1

  • 由来種

    Rabbit

  • 特異性

    This antibody specifically recognizes the 85 kDa fragment of cleaved PARP1 and can be used as marker for detecting apoptotic cells. Cleavage site specific antibody, unconjugated. The antiserum was produced against a chemically synthesized peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP1 and will recognize Asp 214 and Gly 215.

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Human, Recombinant fragment

  • 免疫原

    Synthetic peptide corresponding to Human Cleaved PARP1.
    (Peptide available as ab10779)

  • ポジティブ･コントロール

    • WB: THP1 Nuclear Enriched, HeLa Nuclear Enriched, KARPAS-299 and Daudi cell lysate. HeLa and Jurkat cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab4830の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (4)
1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
特記事項
WB
1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).

ターゲット情報

  • 機能

    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.

  • 配列類似性

    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.

  • 翻訳後修飾

    Phosphorylated by PRKDC and TXK.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.

  • 細胞内局在

    Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ADP ribosyltransferase diphtheria toxin like 1 antibody
      • ADP ribosyltransferase NAD(+) antibody
      • ADP-ribosyltransferase diphtheria toxin-like 1 antibody
      • ADPRT 1 antibody
      • ADPRT antibody
      • ADPRT1 antibody
      • APOPAIN antibody
      • ARTD1 antibody
      • NAD(+) ADP-ribosyltransferase 1 antibody
      • PARP antibody
      • PARP-1 antibody
      • PARP1 antibody
      • PARP1_HUMAN antibody
      • Poly [ADP-ribose] polymerase 1 (PARP-1) antibody
      • Poly [ADP-ribose] polymerase 1 antibody
      • Poly ADP ribose polymerase 1 antibody
      • Poly(ADP ribose) polymerase antibody
      • Poly[ADP-ribose] synthase 1 antibody
      • PPOL antibody
      • SCA1 antibody
      see all

    画像

    • Western blot - Anti-Cleaved PARP1 antibody (ab4830)
      Western blot - Anti-Cleaved PARP1 antibody (ab4830)
      All lanes : Anti-Cleaved PARP1 antibody (ab4830) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Jurkat cells treated with Etoposide (1 µM for 16 hours)
      Lanes 3 & 5 : HeLa cell lysate
      Lane 4 : HeLa cells treated with Etoposide (1 µM for 16 hours)
      Lane 6 : HeLa cells treated with Staurosporine (3 µM for 16 hours)

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/14000 dilution

      Predicted band size: 85 kDa

    • Western blot - Anti-Cleaved PARP1 antibody (ab4830)
      Western blot - Anti-Cleaved PARP1 antibody (ab4830)
      All lanes : Anti-Cleaved PARP1 antibody (ab4830) at 1/2000 dilution

      Lane 1 : THP1 Nuclear Enriched
      Lane 2 : HeLa Nuclear Enriched
      Lane 3 : KARPAS-299
      Lane 4 : Daudi cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution

      Predicted band size: 85 kDa

    • Western blot - Anti-Cleaved PARP1 antibody (ab4830)
      Western blot - Anti-Cleaved PARP1 antibody (ab4830)This image is courtesy of an Abreview submitted by Adam Szadkowski on 26 January 2006.
      All lanes : Anti-Cleaved PARP1 antibody (ab4830) at 1/1000 dilution

      Lane 1 : Non-induced Jurkat cells
      Lane 2 : Induced Jurkat cells

      Secondary
      All lanes : Goat Anti-Rabbit HRP

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 85 kDa
      Observed band size: 85 kDa


      Exposure time: 5 seconds

      See Abreview

    参考文献 (43)

    ab4830 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab4830 は 43 報の論文で使用されています。

    • Jo D  et al. Role of Exendin-4 in Brain Insulin Resistance, Mitochondrial Function, and Neurite Outgrowth in Neurons under Palmitic Acid-Induced Oxidative Stress. Antioxidants (Basel) 10:N/A (2021). PubMed: 33435277
    • Liu Y  et al. Circ_HIPK3 alleviates CoCl2-induced apoptotic injury in neuronal cells by depending on the regulation of the miR-222-3p/DUSP19 axis. Biochem Biophys Res Commun 553:126-133 (2021). PubMed: 33770577
    • Mamriev D  et al. A small-molecule ARTS mimetic promotes apoptosis through degradation of both XIAP and Bcl-2. Cell Death Dis 11:483 (2020). PubMed: 32587235
    • Sun C  et al. FGL1 regulates acquired resistance to Gefitinib by inhibiting apoptosis in non-small cell lung cancer. Respir Res 21:210 (2020). PubMed: 32778129
    • Tu CC  et al. STIM2 knockdown protects against ischemia/reperfusion injury through reducing mitochondrial calcium overload and preserving mitochondrial function. Life Sci 247:116560 (2020). PubMed: 31200000
    View all Publications for this product

    レビューと Q&A

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    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-Cleaved PARP1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Vastus lateralis muscle)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    50 µg
    Specification
    Vastus lateralis muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 04 2014

    Western blot abreview for Anti-Cleaved PARP1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (8% Gel)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Dr. G.K Sakellariou

    Verified customer

    投稿 May 14 2013

    Western blot abreview for Anti-Cleaved PARP1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (glioma cells)
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Loading amount
    20 µg
    Treatment
    0.5 mM staurosporine in DMSO for 6hrs
    Specification
    glioma cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 08 2010

    Western blot abreview for Anti-Cleaved PARP1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Jurkat cells)
    Specification
    Jurkat cells
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Mr. Adam Szadkowski

    Verified customer

    投稿 Jan 26 2006

    Question

    Dear sirs   I would be interested in buying the 2 antibodies listed below and to use them on bovine cells. Since I see that the anti caspase 3 one has not been reviewed yet in this species, would you be interested in getting it tested in bovine?   Anti-Caspase 3 antibody - Cleaved at Asp175 (ab52294) Anti-Cleaved PARP antibody (ab4830)

    Read More
    Answer

    Thank you for contacting Abcam with this inquiry. Unfortunately the homology between the immunogen of ab52294 and bovine Caspase-3 falls below the threshold for qualification for our Abreview testing discount. Please let me know if you have any questions or there are other way in which I might help.

    Read More

    Question

    Dear Sir/Madam The customer would like to PARP antibody that result same with the publication (page 1387, attach file) which have PARP protein and cleave form. So, please kindly recommend abcam antibody.

    Read More
    Answer

    Thank you for contacting us. We have several antibodies which can be used to detect PARP in Western blotting. From the study I believe you wish to detect both the cleaved (C-terminal fragment) and the full length PARP form in mouse samples? This can be done using ab75757 (Chicken polyclonal) or ab37722 (Rabbit polyclonal). Although ab37722 has not been tested with mouse samples due but to the similarity in immunogen sequence (100%) it is predicted to react. If however you are interested in human samples this can be achieved with ab32071 (Rabbit monoclonal [E78]) which was raised against a synthetic peptide of the C-teminal residues of human PARP. Please note this antibody does not react with mouse samples. We also have several antibodies that specifically recognise the cleavage product of PARP such as ab4830 (reacts with human C-terminal PARP) and ab32064 (reacts with mouse and human PARP, N-terminal). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 167107 DESCRIPTION OF THE PROBLEM no band SAMPLE Cell extract PRIMARY ANTIBODY ABCAM/Rabbit/1:1000 and 1:500 dilutions in 5% milk/TBST(Also tried without blocking with milk at all---using TBST alone. overnight incubation DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED ABCAM Beta actin purchased last year used as negative control. ANTIBODY STORAGE CONDITIONS -20 degrees SAMPLE PREPARATION RIPA buffer/PMSF AMOUNT OF PROTEIN LOADED 30 micrograms ELECTROPHORESIS/GEL CONDITIONS 10% gel (acrylamide gel) TRANSFER AND BLOCKING CONDITIONS Transfer buffer: Tris base 48mM--5.8g, Glycine 39mM---2.9g, SDS 0.37g, 20% methanol---200ml to make 1L. Blocking agent: 5% milk. Blocking time: I hour SECONDARY ANTIBODY Santa Cruz/Rabbit/5% milk/TBST/1:1000 Incubation for 3 hours at room temperature HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 5 HAVE YOU RUN A "NO PRIMARY" CONTROL? No

    Read More
    Answer

    I'm sorry to hear you are having a problem with ab4830. I did have a question about the samples you used. What kind of cells did you test? Were these treated to induce apoptosis? This antibody will only detect cleaved PARP, so if the cells are not apoptotic, the protein will not be detected (please see the second Western blot image, where the uninduced cells show no band, and the induced show a very nice band). The rest of the protocol looks fine to me. It may just be that the sample you are using is not supposed to have cleaved PARP. Please let me know if this helps and do not hesitate to contact us for further advice.

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