製品の概要
-
製品名Anti-Cleaved PARP1 antibody
Cleaved PARP1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Cleaved PARP1
-
由来種Rabbit
-
特異性This antibody recognizes only the large fragment of PARP1 (89 kDa) and does not react with full length PARP1. The immunogen does not contain Asp214.
-
アプリケーション適用あり: WB, ICC/IFmore details
-
種交差性交差種: Human
-
免疫原
Synthetic peptide corresponding to Human Cleaved PARP1 (N terminal). N-terminal residues of the catalytic domain of human PARP1.
Database link: NP_001609
法規制情報
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
バッファーPreservative: 0.02% Thimerosal (merthiolate)
Constituents: 50% Glycerol, 1% BSA, PBS -
Concentration information loading... -
精製度Immunogen affinity purified
-
ポリ/モノポリクローナル
-
アイソタイプIgG
-
研究分野
関連製品
-
Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab2317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa. | |
| ICC/IF | Use a concentration of 10 - 20 µg/ml. |
ターゲット情報
-
機能Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
-
配列類似性Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers. -
翻訳後修飾Phosphorylated by PRKDC and TXK.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity. -
細胞内局在Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
- Information by UniProt
-
参照データベース
- Entrez Gene: 142 Human
- Omim: 173870 Human
- SwissProt: P09874 Human
- Unigene: 177766 Human
-
別名
- ADP-ribosyltransferase diphtheria toxin-like 1 antibody
- ADPRT 1 antibody
- ADPRT antibody
see all
参考文献
This product has been referenced in:
- Williams AA et al. Apoptotic Activity of MeCP2 Is Enhanced by C-Terminal Truncating Mutations. PLoS One 11:e0159632 (2016). Read more (PubMed: 27442528) »
- Zhu W et al. Sensing cytosolic RpsL by macrophages induces lysosomal cell death and termination of bacterial infection. PLoS Pathog 11:e1004704 (2015). WB . Read more (PubMed: 25738962) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"