Anti-Cleaved Caspase-9 抗体 (ab2324)

Thank you for contacting us.

I have had a look at our catalogue and the naming of our antibodies is indeed confusing. We work with different collaborators and they have different ways of naming the antibodies. For example, some of them will name the antibody anti-active caspase 9 antibody if it has been proved to recognise the cleaved caspase 9. Others, will simply call the antibody anti-caspase 9 even if it has been shown to recognise the cleaved procaspase 9.

To help you choose a relevant antibody, I have gone through our catalogue and picked out the antibodies which should recognise the cleaved form of caspase 9:

ab133520 (https://www.abcam.com/ab133520)
ab63342 (https://www.abcam.com/ab63342)
ab61129 (https://www.abcam.com/ab61129)

I have also found some antibodies which will recognise the procaspase (46 kDa) or fully cleaved caspase (25 kDa) as well as the cleaved caspase protein (35 kDa):

ab25758 (https://www.abcam.com/ab25758)
ab258131 (https://www.abcam.com/ab258131)
ab115161 (https://www.abcam.com/ab115161)

Alternatively, I have found a lot in Japan of ab2324 different from the one you received. If you would like ab2324 as a replacement, please note that this may take some time to arrive.

I hope this information is helpful to you. Please let me know which resolution you would prefer.

Unfortunately, this product is not covered by our guarantee as it was bought over 6 months ago.

Given that I believe the protocol used should have given satisfactory results and that your customer has spent much time in the lab trying to resolve this problem using the Dotblot test, I will look into making an exception this time and extending our guarantee.

We no longer have this antibody ab2324 in stock so I am unable to offer you a free of charge replacement with another lot. I can however suggest ab77814 (https://www.abcam.com/ab77814) or ab69541 (https://www.abcam.com/ab69541) as replacements. Alternatively, I could issue your customer with a credit note.

Please let me know which outcome your customer would prefer and inform them that this is an exception that I am making and that for the purchase of future products, the guarantee will only apply to products bought within the 6 month period.

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

Having reviewed the protocol details, I believe this product should have given satisfactory results.

I apologise for the inconvenience. According to our records, this product was ordered on 30/06/11 and you received lot GR21477-3. Could you confirm whether this information is correct?

In addition, can I ask how you stored this antibody?

If the product was purchased within the last 6 months, I would be pleased to offer you a free of charge replacement or credit note in compensation.

Thank you for your cooperation. I look forward to hearing from you.

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. Could you also resend the results in a jpeg format as our system does not allow us to read rar files?

This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.


I look forward to receiving your reply.

Thank you for your enquiry. Pro-caspase-9 is an approximately 47 kDa protein that is cleaved at an autoactivation site 315/316 resulting in a 35 kDa fragment and a 12 kDa fragment. ab2324 will recognize the cleaved caspase-9 N-terminal fragment (p35)only. ab4824 will recognize cleavage site 315/316 and the C-terminal fragment (p12) only, and there is a peptide that you can buy for blocking (ab5251) to use as a positive/negative control for this antibody. ab19425 will recognize Pro-Caspase-9 (p47) and may or may not recognize p35. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

The immunogens are synthetic peptides mapping to the N-terminal of the cleavage site: Asp330 (human) and Asp353 (rat). Unfortunately, the exact sequence of the immunogen remains proprietary.