Anti-Cleaved Caspase-9 抗体 (ab2324)
製品の概要
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製品名
Anti-Cleaved Caspase-9 antibody -
製品の詳細
Rabbit polyclonal to Cleaved Caspase-9 -
由来種
Rabbit -
特異性
This antibody recognizes only the cleaved caspase-9 (37 kDa). It does not recognize full-length caspase-9 or any other caspase.
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アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human -
ポジティブ・コントロール
- WB: Etoposide treated Jurkat whole cell lysate.
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特記事項
Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase 9 (47 kDa) and Apaf-1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. This affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
一次抗体 備考
Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase 9 (47 kDa) and Apaf-1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. This affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab2324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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IP | Use a concentration of 10 - 20 µg/ml. | |
WB | Use a concentration of 0.5 - 1 µg/ml. |
ターゲット情報
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参照データベース
- Entrez Gene: 842 Human
- Omim: 602234 Human
- SwissProt: P55211 Human
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別名
- active caspase 9 antibody
- APAF 3 antibody
- APAF3 antibody
see all
画像
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All lanes : Anti-Cleaved Caspase-9 antibody (ab2324)
Lane 1 : Jurkat cell lysate, untreated
Lane 2 : Jurkat cell lysate, treated with etoposide for 6 hours
Observed band size: 37 kDa why is the actual band size different from the predicted?
プロトコール
参考文献
This product has been referenced in:
- Chen Z et al. Protective effects of lncRNA H19 silence against hypoxia-induced injury in PC-12 cells by regulating miR-28. Int J Biol Macromol 121:546-555 (2019). Read more (PubMed: 30312698) »
- Qi D et al. Knockdown of lncRNA-H19 inhibits cell viability, migration and invasion while promotes apoptosis via microRNA-143/RUNX2 axis in retinoblastoma. Biomed Pharmacother 109:798-805 (2019). Read more (PubMed: 30551533) »