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  1. Link

    cleaved-caspase-9-antibody-ab2324.pdf

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Cell Biology Apoptosis Intracellular Caspases etc Caspases
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Anti-Cleaved Caspase-9 抗体 (ab2324)

  • Datasheet
  • SDS
Reviews (4)Q&A (8)References (71)

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  • Western blot - Anti-Cleaved Caspase-9 antibody (ab2324)

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関連製品

  • データシート
  • 文献 (71)
  • プロトコール
医薬用外毒物

製品の概要

  • 製品名

    Anti-Cleaved Caspase-9 antibody
  • 製品の詳細

    Rabbit polyclonal to Cleaved Caspase-9
  • 由来種

    Rabbit
  • 特異性

    This antibody recognizes only the cleaved caspase-9 (37 kDa). It does not recognize full-length caspase-9 or any other caspase.

  • アプリケーション

    適用あり: IP, WBmore details
  • 種交差性

    交差種: Human
  • ポジティブ・コントロール

    • WB: Etoposide treated Jurkat whole cell lysate.
  • 特記事項

    Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase 9 (47 kDa) and Apaf-1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. This affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol, 0.5% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 一次抗体 備考

    Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase 9 (47 kDa) and Apaf-1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. This affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases

関連製品

  • Assay kits

    • Caspase 9 Assay Kit (Colorimetric) (ab65608)
    • Caspase 9 (active) FITC Staining Kit (ab65615)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

Our Abpromise guarantee covers the use of ab2324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml.

ターゲット情報

  • 参照データベース

    • Entrez Gene: 842 Human
    • Omim: 602234 Human
    • SwissProt: P55211 Human
    • 別名

      • active caspase 9 antibody
      • APAF 3 antibody
      • APAF3 antibody
      • Apoptotic protease activating factor 3 antibody
      • Apoptotic protease MCH 6 antibody
      • CASP9 antibody
      • Caspase 9 antibody
      • Caspase 9 apoptosis related cysteine peptidase antibody
      • Cleaved caspase 9 antibody
      • ICE LAP6 antibody
      • ICE like apoptotic protease 6 antibody
      • MCH6 antibody
      • PPP1R56 antibody
      • Protein phosphatase 1 regulatory subunit 56 antibody
      see all

    画像

    • Western blot - Anti-Cleaved Caspase-9 antibody (ab2324)
      Western blot - Anti-Cleaved Caspase-9 antibody (ab2324)
      All lanes : Anti-Cleaved Caspase-9 antibody (ab2324)

      Lane 1 : Jurkat cell lysate, untreated
      Lane 2 : Jurkat cell lysate, treated with etoposide for 6 hours

      Observed band size: 37 kDa
      why is the actual band size different from the predicted?

    プロトコール

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献

    This product has been referenced in:

    • Chen Z  et al. Protective effects of lncRNA H19 silence against hypoxia-induced injury in PC-12 cells by regulating miR-28. Int J Biol Macromol 121:546-555 (2019). Read more (PubMed: 30312698) »
    • Qi D  et al. Knockdown of lncRNA-H19 inhibits cell viability, migration and invasion while promotes apoptosis via microRNA-143/RUNX2 axis in retinoblastoma. Biomed Pharmacother 109:798-805 (2019). Read more (PubMed: 30551533) »
    See all 71 Publications for this product

    ab2324 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 12 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-active Caspase 9 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Methanol
    Permeabilization
    No
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    投稿 Oct 14 2008

    Flow Cytometry abreview for Anti-active Caspase 9 antibody

    Poor
    Abreviews
    Abreviews
    Application
    Flow Cytometry
    Sample
    Human Cell (Peripheral blood mononuclear cell)
    Permeabilization
    Yes - BD Perm/Wash buffer
    Gating Strategy
    lymphocytes
    Specification
    Peripheral blood mononuclear cell
    Preparation
    Cell harvesting/tissue preparation method: Spin down, wash in BD Perm/Wash buffer 1x, fix, wash 2x, and stain with primary antibody 30 minutes.
    Sample buffer: Buffer composition at analysis was < 0.09% sodium azide with FBS in phosphate buffered solution.
    Fixation
    Formaldehyde
    Read More

    Ms. Janine Sabino

    Verified customer

    投稿 Aug 20 2018

    Western blot abreview for Anti-active Caspase 9 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Tissue lysate - whole (liver)
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 26 2014

    Question

    the replaced antibody is https://www.abcam.com/active-Caspase-9-antibody-ab2324.html

    so I searched in your product and found this https://www.abcam.com/caspase-9-antibody-epr5476-ab133520.html or this https://www.abcam.com/Caspase-9-antibody-ab25758.html

    My question is ......according that I need to determine apoptosis and differantiate between apoptotic cells and normal cells in my work ........one of these is called active cleaved caspase 9(ab2324) and the other is caspase 9(the other two antibodies) but both detect the cleaved part so both will detect apoptosis is that write ??? and if so what is the difference between the active caspase and caspase if both detect the cleaved part which indicates apoptosis??

    Read More

    Abcam community

    Verified customer

    Asked on Dec 10 2012

    Answer

    Thank you for contacting us.

    I have had a look at our catalogue and the naming of our antibodies is indeed confusing. We work with different collaborators and they have different ways of naming the antibodies. For example, some of them will name the antibody anti-active caspase 9 antibody if it has been proved to recognise the cleaved caspase 9. Others, will simply call the antibody anti-caspase 9 even if it has been shown to recognise the cleaved procaspase 9.

    To help you choose a relevant antibody, I have gone through our catalogue and picked out the antibodies which should recognise the cleaved form of caspase 9:

    ab133520 (https://www.abcam.com/ab133520)
    ab63342 (https://www.abcam.com/ab63342)
    ab61129 (https://www.abcam.com/ab61129)

    I have also found some antibodies which will recognise the procaspase (46 kDa) or fully cleaved caspase (25 kDa) as well as the cleaved caspase protein (35 kDa):

    ab25758 (https://www.abcam.com/ab25758)
    ab258131 (https://www.abcam.com/ab258131)
    ab115161 (https://www.abcam.com/ab115161)

    Alternatively, I have found a lot in Japan of ab2324 different from the one you received. If you would like ab2324 as a replacement, please note that this may take some time to arrive.

    I hope this information is helpful to you. Please let me know which resolution you would prefer.

    Read More

    Abcam Scientific Support

    Answered on Dec 10 2012

    Question

    Accordingly please note that this item was ordered on 27 June 2011. The customer has confirmed that the vial was stored 9 months on -80C and then -20C until now.

    Please advise what would we do with this customer ?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 05 2012

    Answer

    Unfortunately, this product is not covered by our guarantee as it was bought over 6 months ago.

    Given that I believe the protocol used should have given satisfactory results and that your customer has spent much time in the lab trying to resolve this problem using the Dotblot test, I will look into making an exception this time and extending our guarantee.

    We no longer have this antibody ab2324 in stock so I am unable to offer you a free of charge replacement with another lot. I can however suggest ab77814 (https://www.abcam.com/ab77814) or ab69541 (https://www.abcam.com/ab69541) as replacements. Alternatively, I could issue your customer with a credit note.

    Please let me know which outcome your customer would prefer and inform them that this is an exception that I am making and that for the purchase of future products, the guarantee will only apply to products bought within the 6 month period.

    Read More

    Abcam Scientific Support

    Answered on Dec 05 2012

    Question

    Questionnaire attached to email for WB.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 04 2012

    Answer

    Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

    Having reviewed the protocol details, I believe this product should have given satisfactory results.

    I apologise for the inconvenience. According to our records, this product was ordered on 30/06/11 and you received lot GR21477-3. Could you confirm whether this information is correct?

    In addition, can I ask how you stored this antibody?

    If the product was purchased within the last 6 months, I would be pleased to offer you a free of charge replacement or credit note in compensation.

    Thank you for your cooperation. I look forward to hearing from you.

    Read More

    Abcam Scientific Support

    Answered on Dec 04 2012

    Question


    The complain :-
    That this substance 'Rabbit polyclonal to active Caspase9 ' ab2324

    is barely working and dose not give any result and the attached photo is a Dotblot test which is very faint.

    hope to solve this problem as this is my 1st time to deal with your company .

    Read More

    Abcam community

    Verified customer

    Asked on Nov 29 2012

    Answer

    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. Could you also resend the results in a jpeg format as our system does not allow us to read rar files?

    This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.


    I look forward to receiving your reply.

    Read More

    Abcam Scientific Support

    Answered on Nov 29 2012

    Flow Cytometry abreview for Anti-active Caspase 9 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Jurkat)
    Specification
    Jurkat
    Preparation
    Cell harvesting/tissue preparation method: Spin down, wash in FACS buffer 1x, fix, wash 2x, and stain with primary antibody overnight.
    Sample buffer: Buffer composition at analysis was 0.1% sodium azide with FBS in phosphate buffered solution.
    Fixation
    Paraformaldehyde
    Permeabilization
    Yes - Triton X-100 and NP40
    Gating Strategy
    Isolate cell population from plot of SSC-A / FSA-A
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 15 2011

    Question

    Do you have any antibodies that will allow me to detect both Pro-Caspase-9 and the cleaved activated caspase-9?

    Read More

    Abcam community

    Verified customer

    Asked on May 24 2005

    Answer

    Thank you for your enquiry. Pro-caspase-9 is an approximately 47 kDa protein that is cleaved at an autoactivation site 315/316 resulting in a 35 kDa fragment and a 12 kDa fragment. ab2324 will recognize the cleaved caspase-9 N-terminal fragment (p35)only. ab4824 will recognize cleavage site 315/316 and the C-terminal fragment (p12) only, and there is a peptide that you can buy for blocking (ab5251) to use as a positive/negative control for this antibody. ab19425 will recognize Pro-Caspase-9 (p47) and may or may not recognize p35. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

    Read More

    Abcam Scientific Support

    Answered on May 24 2005

    Question

    Have your active caspase-9 antibodies either human or rat (ab 2324 or ab 2325) been tested on mouse? If not could you tell me which region each antibody recognises so that we can judge whether or not we think it will recognize mouse.

    Read More

    Abcam community

    Verified customer

    Asked on May 27 2004

    Answer

    The immunogens are synthetic peptides mapping to the N-terminal of the cleavage site: Asp330 (human) and Asp353 (rat). Unfortunately, the exact sequence of the immunogen remains proprietary.

    Read More

    Abcam Scientific Support

    Answered on Jun 02 2004

    1-10 of 12 Abreviews or Q&A

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