Anti-Cleaved Caspase-3 抗体 (ab2302)
製品の概要
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製品名
Anti-Cleaved Caspase-3 antibody
Cleaved Caspase-3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cleaved Caspase-3 -
由来種
Rabbit -
特異性
The antibody preferentially recognizes the p17 fragment of the active Caspase-3. Under the conditions we tested, the antibody did not detect the precursor form. Under other conditions (such as those used in PMC2206181), this antibody has been found to detect both the pro and active forms of Caspase-3. Although some customers have used this antibody successfully in mouse and rat, we do not batch test in these species and so cannot guarantee that it will work in them. -
アプリケーション
適用あり: IHC-FoFr, IHC-P, IHC-Fr, WBmore details -
種交差性
交差種: Rabbit, Human, Quail
交差が予測される動物種: Mouse, Rat
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免疫原
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ポジティブ･コントロール
- Camptothecin (2 µM) treated Jurkat cells. If no signal is observed a time course may be required to identify maximal caspase activity.
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特記事項
Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab2302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 18797916 | |
| IHC-P | Use a concentration of 5 - 20 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. | |
| IHC-Fr | Use a concentration of 10 - 20 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa. |
ターゲット情報
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機能
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. -
組織特異性
Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. -
配列類似性
Belongs to the peptidase C14A family. -
翻訳後修飾
Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 836 Human
- Entrez Gene: 12367 Mouse
- Entrez Gene: 25402 Rat
- Omim: 600636 Human
- SwissProt: P42574 Human
- SwissProt: P70677 Mouse
- SwissProt: Q8MJC3 Rabbit
- SwissProt: P55213 Rat
see all -
別名
- active caspase 3 antibody
- Apopain antibody
- CASP 3 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved Caspase-3 antibody (ab2302)IHC image of cleaved caspase-3 staining in human tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2302, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)All lanes : Anti-Cleaved Caspase-3 antibody (ab2302) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate (2 uM Staurosporine, 4Hr) at 20 µg
Lane 2 : HeLa Whole Cell Lysate (untreated) at 20 µg
Lane 3 : Cleaved Caspase 3 (recombinant protein) at 0.1 µg
Secondary
All lanes : 800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Additional bands at: 17 kDa (possible mature (processed) protein), 24 kDa, 35 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved Caspase-3 antibody (ab2302)This image is courtesy of Carl Hobbs, King's College London, United KingdomImmunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of cleaved Caspase-3 antibody (ab2302) on Quail Tissue sections (Quail E6/7 developing DRGs Saggital section). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins RT. Primary Antibody ab2302 incubated at 1/50 for at RT. Secondary Antibody: Biotin conjugated goat anti rabbit IgG (1/300).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved Caspase-3 antibody (ab2302)This image is courtesy of an anonymous Abreviewab2302 staining cleaved Caspase-3 in human ulcerative colitis, colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed using the HOPE method and permeabilized. Samples were incubated with primary antibody (1/20) for 1 hour at 25°C. A Biotin-SP-conjugated donkey anti-rabbit IgG polyclonal (1/800) was used as the secondary antibody.
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Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)Anti-Cleaved Caspase-3 antibody (ab2302) at 1 µg/ml + Camptothecin
(2 µM) treated Jurkat cells. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved Caspase-3 antibody (ab2302)Lung sections were stained with caspase antibody ab2302. Caspase-3 positive cells are shown in brown (DAB). This picture was kindly supplied as part of a customer review.
参考文献
This product has been referenced in:
- Li Y et al. LncRNA-LET relieves hypoxia-induced injury in H9c2 cells through regulation of miR-138. J Cell Biochem 121:259-268 (2020). Read more (PubMed: 31222827) »
- Shi Q et al. microRNA-29a functions as a tumor suppressor in nasopharyngeal carcinoma 5-8F cells through targeting VEGF. Iran J Basic Med Sci 22:541-546 (2019). Read more (PubMed: 31217935) »
