Anti-Claudin 4 抗体 (ab53156)
Key features and details
- Rabbit polyclonal to Claudin 4
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Claudin 4 antibody
Claudin 4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Claudin 4 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Pig -
免疫原
Synthetic peptide within Human Claudin 4 aa 160-209. The exact sequence is proprietary.
Database link: O14493 -
ポジティブ・コントロール
- WB: Untreated HeLa cell extracts, Wild-type MCF7, PC-3, LNCaP and SW480 cell lysates. IHC-P: Human colon tissue. ICC/IF: MCF7 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53156の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | (3) |
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
ICC/IF | (1) |
Use a concentration of 5 µg/ml.
|
WB |
1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
|
特記事項 |
---|
IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 5 µg/ml. |
WB
1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
ターゲット情報
-
機能
Plays a major role in tight junction-specific obliteration of the intercellular space. -
関連疾患
Note=CLDN4 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. -
配列類似性
Belongs to the claudin family. -
細胞内局在
Cell junction > tight junction. Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 1364 Human
- Entrez Gene: 12740 Mouse
- Entrez Gene: 304407 Rat
- Omim: 602909 Human
- SwissProt: O14493 Human
- SwissProt: O35054 Mouse
- Unigene: 647036 Human
- Unigene: 699253 Human
see all -
別名
- Claudin-4 antibody
- Claudin4 antibody
- CLD4_HUMAN antibody
see all
画像
-
All lanes : Anti-Claudin 4 antibody (ab53156) at 1/500 dilution
Lane 1 : Wild-type MCF7 cell lysate
Lane 2 : CLDN4 knockout MCF7 cell lysate
Lane 3 : PC-3 cell lysate
Lane 4 : U-2 OS cell lysate
Lane 5 : LNCaP cell lysate
Lane 6 : SW480 cell lysate
Lane 7 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-Claudin 4 antibody staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab53156 was shown to bind specifically to Claudin 4. A band was observed at 17 kDa in wild-type MCF7 cell lysates with no signal observed at this size in CLDN4 knockout cell line ab274946 (knockout cell lysate ab275004). To generate this image, wild-type and CLDN4 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
-
All lanes : Anti-Claudin 4 antibody (ab53156) at 1/500 dilution
Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts with immunizing peptide
Predicted band size: 22 kDa
Observed band size: 22 kDa -
ab53156 staining Claudin 4 in human colon tissue. Staining is localized to the cell membrane.
Left panel: ab53156 at 4 µg/ml.Right panel: Isotype control.
Sections were stained using an automated system at room temperature. Sections were rehydrated and antigen retrieved with EDTA pH 9.0. Slides were blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked again for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with hematoxylin and coverslipped. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
ICC/IF image of ab53156 staining Claudin 4 (green) in MCF7 (Human breast adenocarcinoma cell line) cells. The cells were fixed in 4% formaldehyde (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53156, 5 µg/ml) overnight at +4°C. The secondary antibody was an Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. An Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (34)
ab53156 は 34 報の論文で使用されています。
- Liebe F et al. Effects of butyrate- on ruminal Ca2+ transport: evidence for the involvement of apically expressed TRPV3 and TRPV4 channels. Pflugers Arch 474:315-342 (2022). PubMed: 35098357
- Ishikawa S et al. Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes. Microscopy (Oxf) 71:152-160 (2022). PubMed: 35289919
- Sun W et al. Highly Pathogenic PRRSV-Infected Alveolar Macrophages Impair the Function of Pulmonary Microvascular Endothelial Cells. Viruses 14:N/A (2022). PubMed: 35336858
- Wang H et al. Gut microbiota is causally associated with poststroke cognitive impairment through lipopolysaccharide and butyrate. J Neuroinflammation 19:76 (2022). PubMed: 35379265
- Groestlinger J et al. Alternaria alternata Mycotoxins Activate the Aryl Hydrocarbon Receptor and Nrf2-ARE Pathway to Alter the Structure and Immune Response of Colon Epithelial Cells. Chem Res Toxicol 35:731-749 (2022). PubMed: 35405071