Anti-Claudin 3 抗体 (ab15102)
Key features and details
- Rabbit polyclonal to Claudin 3
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Chicken, Human, Pig
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Claudin 3 antibody
Claudin 3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Claudin 3 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Chicken, Human, Pig -
免疫原
Synthetic peptide within Mouse Claudin 3 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q9Z0G9 -
特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15102の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (2) |
1/100.
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| IHC-P | (3) |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
ICC/IF
1/100. |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. -
関連疾患
Note=CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. -
配列類似性
Belongs to the claudin family. -
細胞内局在
Cell junction > tight junction. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1365 Human
- Entrez Gene: 12739 Mouse
- Entrez Gene: 65130 Rat
- Omim: 602910 Human
- SwissProt: O15551 Human
- SwissProt: Q9Z0G9 Mouse
- SwissProt: Q63400 Rat
- Unigene: 647023 Human
see all -
別名
- C7orf1 antibody
- Claudin-3 antibody
- Claudin3 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Claudin 3 antibody (ab15102)ICC/IF image of ab15102 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15102, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 3 antibody (ab15102)ab15102 (2µg/ml) staining Claudin 3 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong membrane staining of the mucosal epithelial cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (51)
ab15102 は 51 報の論文で使用されています。
- Li E et al. Mechanisms of deoxynivalenol-induced endocytosis and degradation of tight junction proteins in jejunal IPEC-J2 cells involve selective activation of the MAPK pathways. Arch Toxicol 95:2065-2079 (2021). PubMed: 33847777
- Pang L et al. STAT3 Signalling via the IL-6ST/gp130 Cytokine Receptor Promotes Epithelial Integrity and Intestinal Barrier Function during DSS-Induced Colitis. Biomedicines 9:N/A (2021). PubMed: 33673239
- Schilpp C et al. TGF-ß1 increases permeability of ciliated airway epithelia via redistribution of claudin 3 from tight junction into cell nuclei. Pflugers Arch 473:287-311 (2021). PubMed: 33386991
- Romero-Cordoba SL et al. Comprehensive omic characterization of breast cancer in Mexican-Hispanic women. Nat Commun 12:2245 (2021). PubMed: 33854067
- Wang W et al. NEO100 enables brain delivery of blood?brain barrier impermeable therapeutics. Neuro Oncol 23:63-75 (2021). PubMed: 32877532