Anti-Claudin 1 抗体 [SP128] - BSA and Azide free (ab271884)
![Flow Cytometry - Anti-Claudin 1 antibody [SP128] - BSA and Azide free (ab271884)](https://www.abcam.co.jp/ps/products/271/ab271884/Images/ab271884-2-ab115783215428anticlaudin1antibodysp128flowcytometry.jpg "Flow Cytometry - Anti-Claudin 1 antibody [SP128] - BSA and Azide free (ab271884)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP128] to Claudin 1 - BSA and Azide free
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
製品の概要
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製品名
Anti-Claudin 1 antibody [SP128] - BSA and Azide free
Claudin 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP128] to Claudin 1 - BSA and Azide free -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P Human
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Flow cyt: HepG2 cells. IHC-P: Human liver carcinoma tissue.
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
ab271884 is the carrier-free version of ab115783. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.20
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
SP128 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| Flow Cyt |
Human
|
| IHC-P |
Human
|
| All applications |
Mouse
Rat
Cow
Pig
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).
(Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594). -
組織特異性
Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis. -
関連疾患
Ichthyosis-sclerosing cholangitis neonatal syndrome -
配列類似性
Belongs to the claudin family. -
細胞内局在
Cell junction, tight junction. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 414922 Cow
- Entrez Gene: 9076 Human
- Entrez Gene: 12737 Mouse
- Entrez Gene: 65129 Rat
- Omim: 603718 Human
- SwissProt: Q6L708 Cow
- SwissProt: O95832 Human
- SwissProt: O88551 Mouse
see all -
別名
- Claudin-1 antibody
- Claudin1 antibody
- CLD 1 antibody
see all
画像
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Flow Cytometry - Anti-Claudin 1 antibody [SP128] - BSA and Azide free (ab271884)
Flow cytometric analysis of rabbit anti-Claudin 1 (SP128) antibody ab115783 (1/100) in HepG2 cells (green) compared to negative control of rabbit IgG (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115783).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [SP128] - BSA and Azide free (ab271884)](https://www.abcam.co.jp/ps/products/271/ab271884/Images/ab271884-1-ab115783127922anticlaudin1antibodysp128immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [SP128] - BSA and Azide free (ab271884) -
![Anti-Claudin 1 antibody [SP128] - BSA and Azide free (ab271884)](https://www.abcam.co.jp/ps/products/271/ab271884/Images/ab271884-3-benefits-of-recombinant-antibodies.png)
Anti-Claudin 1 antibody [SP128] - BSA and Azide free (ab271884)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271884 は論文での使用が確認できていません。
