Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Vagus nerve)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Thermo HIER Buffer pH 6.0
Permeabilization
No
Specification
Vagus nerve
Blocking step
DAKO Protein Block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Thermo Antibody Diluent
Dilution
1/50
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Biotinylated SP-conjugated Affinipure Goat Anti-Ra
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Nikki Pelot
Verified customer
投稿 Oct 29 2018