アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

私たちはウェブサイトをできるだけ使いやすくするために、クッキーを使用しています。

クッキー・ポリシーでは、使用するクッキーをオプトアウトする方法について説明しています。

クッキーの設定を変更しないままでいる場合、このポリシーに同意しているとみなされます。

続ける 続ける

お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • 診断と治療のソリューション
    カスタム・ソリューション & パートナーシップ

    あなたの診断法や治療法を発展させるための、カスタム抗体開発およびコマーシャル・パートナーシップ

    私たちと共にカスタム・ソリューションを創造する

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  • ブログ
    アブカム・ブログ

    最新の情報はこちら.

    • キャンペーン
    • 製品情報
    • 技術情報

新型コロナウイルス (COVID-19)に伴う当社の対応について

  1. Link

    clathrin-heavy-chain-antibody-x22-ab2731.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Protein Trafficking Vesicle Transport Coat Proteins
Share by email

Anti-Clathrin heavy chain 抗体 [X22] (ab2731)

  • Datasheet
Reviews (17)Q&A (8)References (65)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)
  • Western blot - Anti-Clathrin heavy chain antibody [X22] (ab2731)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [X22] (ab2731)
  • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [X22] (ab2731)
  • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)
  • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)
  • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin antibody [X22] (ab2731)
  • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)
  • Western blot - Anti-Clathrin heavy chain antibody [X22] (ab2731)

Key features and details

  • Mouse monoclonal [X22] to Clathrin heavy chain
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Cow, Dog, Human, Bird
  • Isotype: IgG1

こちらの製品もご検討ください

一次抗体
Product image
Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)
Biochemical
Product image
NMDA, excitotoxic amino acid (ab120052)
Biochemical
Product image
Pitstop® 2, Novel cell-permeable clathrin inhibitor (ab120687)

関連製品

製品の概要

  • 製品名

    Anti-Clathrin heavy chain antibody [X22]
    Clathrin heavy chain 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [X22] to Clathrin heavy chain
  • 由来種

    Mouse
  • 特異性

    Detects clathrin heavy chain.
  • アプリケーション

    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Cow, Dog, Human, Bird
  • 免疫原

    Full length native protein (purified) corresponding to Human Clathrin heavy chain. Purified human brain clathrin heavy chain.

  • エピトープ

    Electron microscopy and proteolysis mapping demonstrate that binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.
  • ポジティブ・コントロール

    • bovine brain extract
  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    X22
  • アイソタイプ

    IgG1
  • 研究分野

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Caveolae and Clathrin

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Clathrin heavy chain protein (ab112271)

アプリケーション

Our Abpromise guarantee covers the use of ab2731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/1000.
WB 1/500. Detects a band of approximately 180 kDa.
IHC-P 1/100.

ターゲット情報

  • 機能

    Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
  • 配列類似性

    Belongs to the clathrin heavy chain family.
  • 細胞内局在

    Cytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession Q00610 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 281080 Cow
    • Entrez Gene: 480578 Dog
    • Entrez Gene: 1213 Human
    • Entrez Gene: 67300 Mouse
    • Entrez Gene: 54241 Rat
    • Omim: 118955 Human
    • SwissProt: P49951 Cow
    • SwissProt: P53675 Human
    • SwissProt: Q00610 Human
    • SwissProt: Q68FD5 Mouse
    • SwissProt: P11442 Rat
    • Unigene: 491351 Human
    • Unigene: 479526 Mouse
    • Unigene: 3589 Rat
    see all
  • 別名

    • CHC17 antibody
    • Clathrin heavy chain antibody
    • Clathrin heavy chain 1 antibody
    • Clathrin heavy chain 2 antibody
    • Clathrin heavy chain like 1 antibody
    • Clathrin heavy chain on chromosome 17 antibody
    • Clathrin heavy polypeptide antibody
    • CLH 17 antibody
    • CLH 22 antibody
    • CLH-17 antibody
    • CLH1_HUMAN antibody
    • CLH17 antibody
    • CLH22 antibody
    • CLTC antibody
    • CLTCL antibody
    • CLTCL1 antibody
    • CLTCL2 antibody
    • CLTD antibody
    • Hc antibody
    • KIAA0034 antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)
    Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)

    Immunocytochemistry/Immunofluorescence analysis of Clathrin heavy chain shows staining in HeLa cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2731 (1:200) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.

  • Western blot - Anti-Clathrin heavy chain antibody [X22] (ab2731)
    Western blot - Anti-Clathrin heavy chain antibody [X22] (ab2731)
    Anti-Clathrin heavy chain antibody [X22] (ab2731) at 1/300 dilution + Human brain lysates at 25 µg

    Secondary
    HRP-conjugated goat anti-mouse IgG + IgM (H+L)

    Developed using the ECL technique.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [X22] (ab2731)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [X22] (ab2731)
    Immunohistochemistry was performed on normal biopsies of deparaffinized Human colon tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Clathrin Heavy chain ab2731 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)
    Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)

    Immunocytochemistry/Immunofluorescence analysis of Clathrin heavy chain shows staining in NCI-H460 cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2731 (1:200) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [X22] (ab2731)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [X22] (ab2731)
    Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Clathrin Heavy chain ab2731 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)
    Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)

    Immunocytochemistry/Immunofluorescence analysis of Clathrin heavy chain shows staining in U251 cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2731 (1:200) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)
    Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic

    ab2731 staining Clathrin heavy chain - Membrane Vesicle Marker in Rat hypothalamus primary cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/500 in 1% goat serum; 0.1% TX100; PBS) for 16 hours at 4°C. An Alexa Fluor®546-conjugated Goat polyclonal to mouse IgG, dilution 1/500, was used as secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin antibody [X22] (ab2731)
    Immunocytochemistry/ Immunofluorescence - Anti-Clathrin antibody [X22] (ab2731)This image is a courtesy of Vladimir Milenkovic

    ab2731 staining Clathrin heavy chain in pig retinal pigment epithelium (RPE) cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton ×100 and blocked with 5% serum for 20 minutes at 250C. Samples were incubated with primary antibody (1/500: in 0.1% TX100, 1% goat serum, 1X PBS) for 16 hours at 4°C. An Alexa Fluor®546-conjugated goat polyclonal to mouse IgG was used as secondary antibody at 1/500 dilution.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)
    Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)

    Xenopus laevis cytoplasmic egg extract visualized live with primary and secondary antibody addition [red is anti-Clathrin heavy chain X22 (ab2731) with goat anti-mouse Alexa Fluor 568 secondary, green is anti-HIP1R (ab77297) with goat anti-rabbit Alexa Fluor 488 secondary]. Large red structures are probably aggregates, but the small structures appear to be specific for vesicle staining.

    See Abreview

  • Western blot - Anti-Clathrin heavy chain antibody [X22] (ab2731)
    Western blot - Anti-Clathrin heavy chain antibody [X22] (ab2731)
    All lanes : Anti-Clathrin heavy chain antibody [X22] (ab2731) at 1/500 dilution

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size: 180 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 240 kDa, 450 kDa. We are unsure as to the identity of these extra bands.

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
  • 参考文献 (65)

    ab2731 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2731 は 65 報の論文で使用されています。

    • Ren F  et al. NRSN2 promotes breast cancer metastasis by activating PI3K/AKT/mTOR and NF-?B signaling pathways. Oncol Lett 19:813-823 (2020). PubMed: 31885716
    • Taverner A  et al. Cholix protein domain I functions as a carrier element for efficient apical to basal epithelial transcytosis. Tissue Barriers 8:1710429 (2020). PubMed: 31928299
    • Moshkanbaryans L  et al. The interaction of assembly protein AP180 and clathrin is inhibited by multi-site phospho-mimetics. Neurochem Int 129:104474 (2019). PubMed: 31129113
    • Cohen EAK  et al. Resolution limit of image analysis algorithms. Nat Commun 10:793 (2019). PubMed: 30770826
    • Berndt-Paetz M  et al. IC/BPS-associated alterations of M2 and M3 muscarinic acetylcholine receptor trafficking in human detrusor. Neurourol Urodyn 38:1818-1827 (2019). PubMed: 31301091
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 25 Abreviews or Q&A

    Question

    We are doing some IF research on frozen rat brains and have always been very satisfied when purchasing antibodies from your company. However the Clathrin antibody is posing some difficulties that we haven't encountered before. When we look at the stained slides the we can see the antibody but it seems to almost have diffused out in the mounting medium as well as we find it all over the slides and not only on the tissue. Is this a known problem with this antibody and can it be sorted out by changing something? The frozen slides become fixated in 4% PFA for 1 hour at first before pre-blocking. The pre-blocking step is performed with 2,5% horseserum in PBS for one hour.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 18 2013

    Answer

    We have never experienced this problem with this antibody. Please note, that this antibody has not been tested in immunohistochemistry on frozen tissue sections and is therefore not covered by the abpromise guarantee.

    This antibody has been used on paraffin slides at a dilution of 1:100, therefore, I would suggest using a similar concentration for IHC-Fr. Since the antibody is diluted down, it is more likely that the problem is due to the dilution buffer than the antibody itself. We have in the past got very successful results using 3% BSA-PBS as the diluent so I would suggest trying this.

    Read More

    Abcam Scientific Support

    Answered on Feb 18 2013

    Question

    Inquiry: To whom it may concern, I am writing you to recorrect my previously sent email address. This is the proper email for which I am waiting your answer. I I would like to inquire about Anti-Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731). Could you ship it to Hungary? Would you be so kind to send a preformal invoice to the above email address with the price of product and shipping? Thank you for your help in advance. Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 30 2012

    Answer

    Thank you for contacting us.

    I sent an proforma with the number 48311 for the Anti-Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731). Please read our offer regarding the RabMab antibodies at the end of this email, it might be useful for you.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Nov 30 2012

    Question

    Inquiry: Dear Sir/Madam, I am planning a large scale experiment using one for the following antibodies but I need to test a small amount from each before ordering large scale. I was wondering if you supply sample free stocks I could test? Abcam ab6323 Anti-beta COP antibody [maD] (ab6323) Abcam ab99552 Anti-SEC23 antibody (ab99552) Abcam ab2731 Anti-Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731) Thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 11 2012

    Answer

    Thank you for contacting us. Because we carry over 95,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews



    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Sep 11 2012

    Western blot abreview for Anti-Clathrin antibody [X22] - Membrane Vesicle Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    African Green Monkey Cell lysate - other (CV1 cells)
    Loading amount
    10 µg
    Specification
    CV1 cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Mr. Nir Drayman

    Verified customer

    投稿 Sep 11 2012

    Immunocytochemistry abreview for Anti-Clathrin antibody [X22] - Membrane Vesicle Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (RAW 264.7)
    Specification
    RAW 264.7
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton-X100 in 2% BSA for 15min
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Read More

    Dr. Mahesh Shivananjappa

    Verified customer

    投稿 May 24 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Clathrin antibody [X22] - Membrane Vesicle Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Xenopus laevis Cell (Xenopus laevis cytoplasmic egg extract)
    Specification
    Xenopus laevis cytoplasmic egg extract
    Fixative
    live, no fixative
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 17 2012

    Western blot abreview for Anti-Clathrin antibody [X22] - Membrane Vesicle Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Xenopus laevis Cell lysate - other (Xenopus laevis cytoplasmic egg extract)
    Loading amount
    40 µg
    Specification
    Xenopus laevis cytoplasmic egg extract
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 17 2012

    Question

    infos sur des marqueurs fluo des voies d'internalisation.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 01 2012

    Answer



    Nous avons dans notre catalogue des anticorps marqueurs des voies d'internalisation tels que les anti-Clathrin ab2731 (https://www.abcam.com/ab2731) et ab59710 (https://www.abcam.com/ab59710). Malheureusement nous n'avons pas ces anticorps couplés à un fluorochrome.

    Nous avons cependant des kits de conjugaison, les kits EasyLink, qui permettent de coupler rapidement et facilement les anticorps primaire. Pour plus d'informations sur ces kits je vous invite à visiter la page suivante : https://www.abcam.com/easylink.

    Pour les voies d'internalisation je vous suggère le lien suivant : https://www.abcam.com/index.html?pageconfig=resource&rid=10236&pid=14.

    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Read More

    Abcam Scientific Support

    Answered on Feb 01 2012

    Western blot abreview for Anti-Clathrin antibody [X22] - Membrane Vesicle Marker

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Platelets)
    Loading amount
    20 µg
    Specification
    Platelets
    Treatment
    ADP for 30 min
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Mahesh Shivananjappa

    Verified customer

    投稿 Nov 24 2011

    Question

    thank you for your reply. Our customer would like to know if you have any abreview for both or for just one these batches about WB analysis. If so, please send me all the information you have.   Thanks in advance Kind regards  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 03 2011

    Answer

    Thank you for your reply. All Abreviews we have are from previous batches of this antibody. This antibody is very popular and sells fast. Therefore even the experiment shown in the latest Abreview was performed with a different batch. I am sorry I cannot provide you with this information, but I would like to reassure you and your customer that at Abcam our products are covered by the Abpromise. Our Abpromise® ensures that you can trust our products to perform as stated on the datasheets, or we will offer a replacement, credit, or refund. The Abcam Abpromise® guarantee: • 100% Scientific and Customer Support for any product you buy from Abcam or one of our authorised distributors. • We guarantee our products work in the tested species and applications stated on the datasheet. • We will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase. • We investigate any quality concerns raised by customers to ensure our catalog contains products that perform to the highest standards. Please note these conditions to our Abpromise®: • Protocol information must be provided in order for the claim to be validated. • Antibodies tested in recombinant samples only are not guaranteed for use on endogenous samples. • “Predicted to react” information on the datasheets is provided for reference only and these species are not guaranteed. • Fast Track antibodies are covered for ELISA against the immunizing peptide only. I hope this information is helpful, but please do not hesitate to contact us with further questions.

    Read More

    Abcam Scientific Support

    Answered on Oct 03 2011

    1-10 of 25 Abreviews or Q&A

    •  戻る
    • 1
    • 2
    • 3
    • 次へ 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    ブログ

    ブログを読む

    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2021 Abcam plc. All rights reserved.