製品の概要

  • 製品名
    Anti-Clathrin heavy chain antibody [TD.1]
    Clathrin heavy chain 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [TD.1] to Clathrin heavy chain
  • 由来種
    Mouse
  • 特異性
    This antibody reacts with clathrin heavy chains (CHC).
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Cow, Human
  • 免疫原

    This antibody was raised using the globular N terminal domain of clathrin heavy chain.

  • ポジティブ･コントロール
    • Methanol fixed HeLa cells for IF.

法規制情報

医薬用外毒物

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 21571834
WB 1/250. Predicted molecular weight: 192 kDa.
ICC/IF Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for IP.

    • ターゲット情報

    • 機能
      Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
    • 配列類似性
      Belongs to the clathrin heavy chain family.
    • 細胞内局在
      Cytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 参照データベース
      • 別名
        • CHC17 antibody
        • Clathrin heavy chain antibody
        • Clathrin heavy chain 1 antibody
        • Clathrin heavy chain 2 antibody
        • Clathrin heavy chain like 1 antibody
        • Clathrin heavy chain on chromosome 17 antibody
        • Clathrin heavy polypeptide antibody
        • CLH 17 antibody
        • CLH 22 antibody
        • CLH-17 antibody
        • CLH1_HUMAN antibody
        • CLH17 antibody
        • CLH22 antibody
        • CLTC antibody
        • CLTCL antibody
        • CLTCL1 antibody
        • CLTCL2 antibody
        • CLTD antibody
        • Hc antibody
        • KIAA0034 antibody
        see all

      画像

      • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [TD.1] (ab24578)
        Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [TD.1] (ab24578)
        ab24578 at a 1/200 dilution staining clathrin triskelion (blue) throughout the methanol fixed HeLa cells by Immunocytochemistry/Immunofluorescence. The Golgi is brilliantly stained by Giantin (yellow). Secondary antibodies used were a goat anti-mouse alexafluor AF546 and a goat anti-rabbit alexafluor AF488 applied at 1 ug/mL. The DNA was visualized with DAPI at 1 ug/mL. The colors of this image were altered.

      参考文献

      This product has been referenced in:
      • Kitahara H  et al. COP35, a cholangiocarcinoma-binding oligopeptide, interacts with the clathrin heavy chain accompanied by GRP78. Mol Cancer Res 9:688-701 (2011). IHC-P ; Human . Read more (PubMed: 21571834) »
      • Harel A  et al. Evidence for CALM in directing VAMP2 trafficking. Traffic 9:417-29 (2008). Read more (PubMed: 18182011) »

      See all 5 Publications for this product

      ab24578 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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