Anti-Clathrin heavy chain 抗体 [TD.1] (ab24578)
![Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [TD.1] (ab24578)](https://www.abcam.co.jp/ps/products/24/ab24578/Images/ab24578-35349-anti-clathrin-heavy-chain-antibody-td1-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [TD.1] (ab24578)")
Key features and details
- Mouse monoclonal [TD.1] to Clathrin heavy chain
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Clathrin heavy chain antibody [TD.1]
Clathrin heavy chain 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [TD.1] to Clathrin heavy chain -
由来種
Mouse -
特異性
This antibody reacts with clathrin heavy chains (CHC). -
アプリケーション
適用あり: ICC/IFmore details
適用なし: IP -
種交差性
交差種: Human -
免疫原
This antibody was raised using the globular N terminal domain of clathrin heavy chain.
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ポジティブ･コントロール
- Methanol fixed HeLa cells for IF.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.03% Thimerosal (merthiolate) -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
TD.1 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24578の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
追加情報
Is unsuitable for IP.
ターゲット情報
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機能
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. -
配列類似性
Belongs to the clathrin heavy chain family. -
細胞内局在
Cytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 1213 Human
- Omim: 118955 Human
- SwissProt: P53675 Human
- SwissProt: Q00610 Human
- Unigene: 491351 Human
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別名
- CHC17 antibody
- Clathrin heavy chain antibody
- Clathrin heavy chain 1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [TD.1] (ab24578)ab24578 at a 1/200 dilution staining clathrin triskelion (blue) throughout the methanol fixed HeLa cells by Immunocytochemistry/Immunofluorescence. The Golgi is brilliantly stained by Giantin (yellow). Secondary antibodies used were a goat anti-mouse alexafluor AF546 and a goat anti-rabbit alexafluor AF488 applied at 1 ug/mL. The DNA was visualized with DAPI at 1 ug/mL. The colors of this image were altered.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab24578 は 9 報の論文で使用されています。
- Mukenhirn M et al. Role of Clathrin Light Chains in Regulating Invadopodia Formation. Cells 10:N/A (2021). PubMed: 33672612
- Pechstein A et al. Vesicle Clustering in a Living Synapse Depends on a Synapsin Region that Mediates Phase Separation. Cell Rep 30:2594-2602.e3 (2020). PubMed: 32101738
- Vassilopoulos S et al. Actin scaffolding by clathrin heavy chain is required for skeletal muscle sarcomere organization. J Cell Biol 205:377-93 (2014). PubMed: 24798732
- Chowdhury D et al. Tyrosine phosphorylation regulates the endocytosis and surface expression of GluN3A-containing NMDA receptors. J Neurosci 33:4151-64 (2013). PubMed: 23447623
- Kitahara H et al. COP35, a cholangiocarcinoma-binding oligopeptide, interacts with the clathrin heavy chain accompanied by GRP78. Mol Cancer Res 9:688-701 (2011). IHC-P ; Human . PubMed: 21571834
- Harel A et al. Evidence for CALM in directing VAMP2 trafficking. Traffic 9:417-29 (2008). PubMed: 18182011
- Praznikar ZJ et al. A presynaptically toxic secreted phospholipase A2 is internalized into motoneuron-like cells where it is rapidly translocated into the cytosol. Biochim Biophys Acta 1783:1129-39 (2008). PubMed: 18261469
- Bushlin I et al. Clathrin assembly protein AP180 and CALM differentially control axogenesis and dendrite outgrowth in embryonic hippocampal neurons. J Neurosci 28:10257-71 (2008). PubMed: 18842885
- Borlido J et al. Clathrin is spindle-associated but not essential for mitosis. PLoS ONE 3:e3115 (2008). WB ; Human . PubMed: 18769625