Anti-Clathrin heavy chain 抗体 [TD.1] (ab24578)
製品の概要
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製品名Anti-Clathrin heavy chain antibody [TD.1]
Clathrin heavy chain 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [TD.1] to Clathrin heavy chain
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由来種Mouse
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特異性This antibody reacts with clathrin heavy chains (CHC).
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アプリケーション適用あり: IHC-P, WB, ICC/IFmore details
適用なし: IP -
種交差性交差種: Cow, Human
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免疫原
This antibody was raised using the globular N terminal domain of clathrin heavy chain.
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ポジティブ･コントロール
- Methanol fixed HeLa cells for IF.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.03% Thimerosal (merthiolate)
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Concentration information loading... -
精製度Protein G purified
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ポリ/モノモノクローナル
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クローン名TD.1
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アイソタイプIgG1
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研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab24578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. PubMed: 21571834 | |
| WB | 1/250. Predicted molecular weight: 192 kDa. | |
| ICC/IF | Use at an assay dependent concentration. |
追加情報
Is unsuitable for IP.
ターゲット情報
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機能Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
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配列類似性Belongs to the clathrin heavy chain family.
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細胞内局在Cytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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参照データベース
- Entrez Gene: 281080 Cow
- Entrez Gene: 1213 Human
- Omim: 118955 Human
- SwissProt: P49951 Cow
- SwissProt: P53675 Human
- SwissProt: Q00610 Human
- Unigene: 491351 Human
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別名
- CHC17 antibody
- Clathrin heavy chain antibody
- Clathrin heavy chain 1 antibody
see all
画像
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![Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [TD.1] (ab24578)](https://www.abcam.co.jp/ps/products/24/ab24578/Images/ab24578_1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [TD.1] (ab24578)ab24578 at a 1/200 dilution staining clathrin triskelion (blue) throughout the methanol fixed HeLa cells by Immunocytochemistry/Immunofluorescence. The Golgi is brilliantly stained by Giantin (yellow). Secondary antibodies used were a goat anti-mouse alexafluor AF546 and a goat anti-rabbit alexafluor AF488 applied at 1 ug/mL. The DNA was visualized with DAPI at 1 ug/mL. The colors of this image were altered.
プロトコール
参考文献
This product has been referenced in:
- Kitahara H et al. COP35, a cholangiocarcinoma-binding oligopeptide, interacts with the clathrin heavy chain accompanied by GRP78. Mol Cancer Res 9:688-701 (2011). IHC-P ; Human . Read more (PubMed: 21571834) »
- Harel A et al. Evidence for CALM in directing VAMP2 trafficking. Traffic 9:417-29 (2008). Read more (PubMed: 18182011) »
