Anti-Claspin 抗体 (ab3721)

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Western blot - Anti-Claspin antibody (ab3721)

    Key features and details

    • Rabbit polyclonal to Claspin
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-Claspin antibody
      Claspin 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to Claspin

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Chimpanzee, Gorilla

    • 免疫原

      Synthetic peptide (Human) - which represents a portion of human Claspin encoded within exon 15.

    • ポジティブ･コントロール

      • HeLa cell lysate.

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab3721 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB 1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa).

    ターゲット情報

    • 機能

      Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.

    • 配列類似性

      Belongs to the claspin family.

    • ドメイン

      The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.

    • 翻訳後修飾

      Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
      Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.

    • 細胞内局在

      Nucleus.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Claspin antibody
        • claspin homolog (Xenopus laevis) antibody
        • Claspin homolog antibody
        • CLSPN antibody
        • CLSPN protein antibody
        • CLSPN_HUMAN antibody
        • hClaspin antibody
        • Hu Claspin antibody
        see all

      画像

      • Western blot - Anti-Claspin antibody (ab3721)
        Western blot - Anti-Claspin antibody (ab3721)

        Sample: Hela cell lysate (20µg).

        Antibody: Affinity purified Rabbit anti-Claspin (ab3721) used at 1:1000 or 1:5000 dilution with (+) or without (-) blocking peptide.

        Detection: ECL.

        Sample: Hela cell lysate (20µg).

        Antibody: Affinity purified Rabbit anti-Claspin (ab3721) used at 1:1000 or 1:5000 dilution with (+) or without (-) blocking peptide.

        Detection: ECL.

      参考文献 (1)

      ab3721 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab3721 は 1 報の論文で使用されています。

      • Jiang K  et al. Regulation of Chk1 includes chromatin association and 14-3-3 binding following phosphorylation on Ser-345. J Biol Chem 278:25207-17 (2003). PubMed: 12676962

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