Anti-Claspin 抗体 (ab3721)

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Immunoprecipitation - Anti-Claspin antibody (ab3721)
  • Western blot - Anti-Claspin antibody (ab3721)

Key features and details

  • Rabbit polyclonal to Claspin
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Claspin antibody
    Claspin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Claspin

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla

  • 免疫原

    Synthetic peptide (Human) - which represents a portion of human Claspin encoded within exon 15.

  • ポジティブ･コントロール

    • HeLa cell lysate.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3721の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa).
IP
Use at 2-10 µg/mg of lysate.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa).
IP
Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 機能

    Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.

  • 配列類似性

    Belongs to the claspin family.

  • ドメイン

    The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.

  • 翻訳後修飾

    Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Claspin antibody
      • claspin homolog (Xenopus laevis) antibody
      • Claspin homolog antibody
      • CLSPN antibody
      • CLSPN protein antibody
      • CLSPN_HUMAN antibody
      • hClaspin antibody
      • Hu Claspin antibody
      see all

    画像

    • Immunoprecipitation - Anti-Claspin antibody (ab3721)
      Immunoprecipitation - Anti-Claspin antibody (ab3721)

      Claspin was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab3721 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab3721 at 0.1µg/mL.

      Lane 1: ab3721 IP in HeLa whole cell lysate.

      Lane 2: ab3721 IP in HeLa whole cell lysate.

      Lane 3: Control IgG in HeLa whole cell lysate.

      Detection: Chemiluminescence with an exposure time of 10 seconds

    • Western blot - Anti-Claspin antibody (ab3721)
      Western blot - Anti-Claspin antibody (ab3721)
      All lanes : Anti-Claspin antibody (ab3721) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : HEK293T whole cell lysate at 50 µg

      Predicted band size: 150 kDa


      Exposure time: 30 seconds

    参考文献 (1)

    ab3721 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3721 は 1 報の論文で使用されています。

    • Jiang K  et al. Regulation of Chk1 includes chromatin association and 14-3-3 binding following phosphorylation on Ser-345. J Biol Chem 278:25207-17 (2003). PubMed: 12676962

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