Application
Western blot
Sample
Cow Cell lysate - whole cell (BoMAC (SV40-transformed bovine macrophages) and HE)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
15 µg
Specification
BoMAC (SV40-transformed bovine macrophages) and HE
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 18°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: HRP-conjugated goat anti-rat
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 140 kDa Non-specific: 65 kDa
Positive control
1st lane = HEK293T cells (human)
2nd lane = bovine macrophages
Additional data
Additional Notes
1st lane = HEK293T cells (human)
2nd lane = bovine macrophages
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Kerry Woods
Verified customer
投稿 Oct 26 2015