Anti-Citrate synthetase 抗体 [EPR8066] - BSA and Azide free (ab248292)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8066] to Citrate synthetase - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Citrate synthetase antibody [EPR8066] - BSA and Azide free
Citrate synthetase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8066] to Citrate synthetase - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details
適用なし: Flow Cyt -
種交差性
交差種: Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab248292 is the carrier-free version of ab129088.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR8066 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Assay kits
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248292の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa).
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa). |
ターゲット情報
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パスウェイ
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. -
配列類似性
Belongs to the citrate synthase family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 1431 Human
- Entrez Gene: 170587 Rat
- Omim: 118950 Human
- SwissProt: O75390 Human
- SwissProt: Q8VHF5 Rat
- Unigene: 430606 Human
- Unigene: 728168 Human
- Unigene: 66581 Rat
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別名
- CISY_HUMAN antibody
- Citrate synthase antibody
- Citrate synthase, mitochondrial antibody
see all
画像
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All lanes : Anti-Citrate synthetase antibody [EPR8066] (ab129088) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Caco-2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 51 kDaThis data was developed using ab129088, the same antibody clone in a different buffer formulation.
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This data was developed using ab129088, the same antibody clone in a different buffer formulation.ab129088, at a dilution of 1/100, staining Citrate synthetase in paraffin-embedded Human kidney tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248292 は論文での使用が確認できていません。