Application
Western blot
Sample
Rat Cell lysate - whole cell (L6 myotubes)
Loading amount
20 µg
Specification
L6 myotubes
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2%BSA in TBST
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/50000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 50 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 23 2011