Anti-Citrate synthetase 抗体 (ab96600)
Key features and details
- Rabbit polyclonal to Citrate synthetase
- Suitable for: IHC-P, ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human, Zebrafish
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Citrate synthetase antibody
Citrate synthetase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Citrate synthetase -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, IP, WBmore details -
種交差性
交差種: Mouse, Rat, Human, Zebrafish
交差が予測される動物種: Cow, Pig, Xenopus laevis -
免疫原
Recombinant fragment within Human Citrate synthetase aa 44-316. The exact sequence is proprietary. NP_938083
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ポジティブ・コントロール
- WB: HEK-293T, A431, HeLa and HepG2 whole cell lysate (ab7900) and zebrafish skeletal muscle tissue and mouse liver mitochondrial homogenates. ICC/IF: HeLa and U-2 OS cells. IHC-P: Human colon, mouse intestine, mouse muscle and NCI-N87 xenograft tissues. IP: HEK-293T whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96600の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
Use at an assay dependent concentration.
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ICC/IF | (1) |
1/100 - 1/1000.
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IP |
1/100 - 1/500.
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WB | (8) |
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/500. |
WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa. |
ターゲット情報
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パスウェイ
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. -
配列類似性
Belongs to the citrate synthase family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 280682 Cow
- Entrez Gene: 1431 Human
- Entrez Gene: 12974 Mouse
- Entrez Gene: 397519 Pig
- Entrez Gene: 170587 Rat
- Entrez Gene: 379886 Xenopus laevis
- Entrez Gene: 322339 Zebrafish
- Omim: 118950 Human
see all -
別名
- CISY_HUMAN antibody
- Citrate synthase antibody
- Citrate synthase, mitochondrial antibody
see all
画像
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All lanes : Anti-Citrate synthetase antibody (ab96600) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 52 kDa10% SDS-PAGE.
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Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Citrate synthetase (green) with ab96600 at a dilution of 1/200.
Cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. Alpha Tubulin (red) was stained with ab184613 at a dilution of 1/1000. Blue: Hoechst 33342 staining.
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Anti-Citrate synthetase antibody (ab96600) at 1/1000 dilution + Rat muscle tissue extracts at 50 µg
Predicted band size: 52 kDa10% SDS-PAGE.
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Anti-Citrate synthetase antibody (ab96600) at 1/1000 dilution + Rat brain tissue extracts at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 52 kDa10% SDS-PAGE.
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Anti-Citrate synthetase antibody (ab96600) at 1/1000 dilution + Mouse brain tissue extracts at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 52 kDa10% SDS-PAGE.
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Immunocytochemistry/Immunofluorescence analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells labelling Citrate synthetase (green) with ab96600 at a dilution of 1/500.
Cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. Alpha Tubulin (red) was stained with ab184613 at a dilution of 1/1000. Blue: Hoechst 33342 staining.
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IHC image of ab96600 staining in human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96600, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunoprecipitation of Citrate synthetase from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate using 5 μg of ab96600.
Western blot analysis was performed using ab96600.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse intestine tissue labeling Citrate synthetase with ab96600 at a dilution of 1/500.
Antigen Retrieval: EDTA based buffer, 15 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse muscle tissue labeling Citrate synthetase with ab96600 at a dilution of 1/500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of NCI-N87 xenograft tissue labeling Citrate synthetase with ab96600 at a dilution of 1/500.
Antigen Retrieval: EDTA based buffer, 15 mins.
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All lanes : Anti-Citrate synthetase antibody (ab96600) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish liver homogenate (20ug)
Lane 3 : Zebrafish skeletal muscle homogenate (20ug)
Lane 4 : Mouse liver mitochondrial homogenate (20ug)
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (139)
ab96600 は 139 報の論文で使用されています。
- Dominiak K et al. Effects of Endurance Training on the Coenzyme Q Redox State in Rat Heart, Liver, and Brain at the Tissue and Mitochondrial Levels: Implications for Reactive Oxygen Species Formation and Respiratory Chain Remodeling. Int J Mol Sci 23:N/A (2022). PubMed: 35055078
- Triolo M et al. Time-dependent changes in autophagy, mitophagy and lysosomes in skeletal muscle during denervation-induced disuse. J Physiol 600:1683-1701 (2022). PubMed: 35067920
- Jessen S et al. Muscle hypertrophic effect of inhaled beta2 -agonist is associated with augmented insulin-stimulated whole-body glucose disposal in young men. J Physiol 600:2345-2357 (2022). PubMed: 35218559
- Almquist NW et al. No Differences Between 12 Weeks of Block- vs. Traditional-Periodized Training in Performance Adaptations in Trained Cyclists. Front Physiol 13:837634 (2022). PubMed: 35299664
- Lv X et al. Tcap Deficiency in Zebrafish Leads to ROS Production and Mitophagy, and Idebenone Improves its Phenotypes. Front Cell Dev Biol 10:836464 (2022). PubMed: 35372370