Anti-Cip4 抗体 (ab72220)
Key features and details
- Rabbit polyclonal to Cip4
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cip4 antibody
Cip4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cip4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant -
免疫原
A region between residues 551 and 601 of human Cip4
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ポジティブ・コントロール
- HeLa cytoplasmic or membrane lysate 293T cytoplasmic or membrane lysate Mouse NIH3T3 cytoplasmic or membrane lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab72220 was affinity purified using an epitope specific to Cip4 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72220の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL. -
組織特異性
Expressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus and trachea. -
配列類似性
Belongs to the FNBP1 family.
Contains 1 FCH domain.
Contains 1 REM (Hr1) repeat.
Contains 1 SH3 domain. -
翻訳後修飾
Tyrosine phosphorylated. Also phosphorylated by PKA. -
細胞内局在
Cytoplasm > perinuclear region and Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Lysosome. Golgi apparatus. Cell membrane. Cell projection > phagocytic cup. Translocates to the plasma membrane in response to insulin stimulation, and this may require active RHOQ (By similarity). Localizes to cortical regions coincident with F-actin, to lysosomes and to sites of phagocytosis in macrophages. Also localizes to the Golgi, and this requires AKAP9. - Information by UniProt
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参照データベース
- Entrez Gene: 9322 Human
- Entrez Gene: 106628 Mouse
- Entrez Gene: 100172170 Orangutan
- Entrez Gene: 116717 Rat
- Omim: 604504 Human
- SwissProt: Q15642 Human
- SwissProt: Q8CJ53 Mouse
- SwissProt: Q5RCJ1 Orangutan
see all -
別名
- Cdc42 interacting protein 4 antibody
- Cdc42 interaction protein 4 long isoform antibody
- Cdc42-interacting protein 4 antibody
see all
画像
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All lanes : Anti-Cip4 antibody (ab72220) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 63 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
Detection of Human Cip4 by Immunoprecipitation. 1mg HeLa whole cell lysate loaded in lanes 1-3 with an antibody recognising an upstream epitope in lane 1, ab72220 at 3 µg in lane 2 and control IgG in lane 3. Image shows immunoprecipitated Cip4 detected using post IP WB, loading 20% of IP and using ab72220 at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab72220 は 1 報の論文で使用されています。
- Zhu YC et al. Suppression of CIP4/Par6 attenuates TGF-ß1-induced epithelial-mesenchymal transition in NRK-52E cells. Int J Mol Med 40:1165-1171 (2017). PubMed: 28848997