保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab117598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 123 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
機能Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription; the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: can phosphorylate the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (in vitro).
関連疾患Bare lymphocyte syndrome 2
配列類似性Contains 4 LRR (leucine-rich) repeats.
Contains 1 NACHT domain.
ドメインThe acetyltransferase domain is necessary for activation of both class I and class II transcription.
The GTP-binding motif doesn't confer GTPase activity but promotes nuclear localization.
翻訳後修飾Autophosphorylated, affecting interaction with TAF7.
細胞内局在Nucleus. Nucleus, PML body. Recruited to PML body by PML.
- Information by UniProt
- C2TA antibody
- C2TA_HUMAN antibody
- Ciita antibody
ab117598 has not yet been referenced specifically in any publications.