Anti-CIITA 抗体 (ab117598)
Key features and details
- Rabbit polyclonal to CIITA
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CIITA antibody
CIITA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CIITA -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide from N terminal region of Human CIITA conjugated to KLH
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ポジティブ・コントロール
- Human tonsil tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117598の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription; the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: can phosphorylate the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (in vitro). -
関連疾患
Bare lymphocyte syndrome 2 -
配列類似性
Contains 4 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
ドメイン
The acetyltransferase domain is necessary for activation of both class I and class II transcription.
The GTP-binding motif doesn't confer GTPase activity but promotes nuclear localization. -
翻訳後修飾
Autophosphorylated, affecting interaction with TAF7. -
細胞内局在
Nucleus. Nucleus, PML body. Recruited to PML body by PML. - Information by UniProt
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参照データベース
- Entrez Gene: 4261 Human
- Entrez Gene: 12265 Mouse
- Entrez Gene: 85483 Rat
- Omim: 600005 Human
- SwissProt: P33076 Human
- SwissProt: P79621 Mouse
- Unigene: 701991 Human
- Unigene: 249560 Mouse
see all -
別名
- C2TA antibody
- C2TA_HUMAN antibody
- CIITA antibody
see all
画像
データシートおよび資料
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Datasheet download
参考文献 (2)
ab117598 は 2 報の論文で使用されています。
- Sæterstad S et al. Profound gene expression changes in the epithelial monolayer of active ulcerative colitis and Crohn's disease. PLoS One 17:e0265189 (2022). PubMed: 35275975
- Zhu D et al. Major histocompatibility complexes are up-regulated in glomerular endothelial cells via activation of c-Jun N-terminal kinase in 5/6 nephrectomy mice. Br J Pharmacol 177:5131-5147 (2020). PubMed: 32830316