Anti-CIDE A 抗体 (ab8402)
Key features and details
- Rabbit polyclonal to CIDE A
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-CIDE A antibody
CIDE A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CIDE A -
由来種
Rabbit -
特異性
Does not recognise CIDE-B. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse -
免疫原
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ポジティブ･コントロール
- Tissue lysate of murine heart. An approximately 25 kDa band can be detected.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8402の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 25 kDa.
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| ICC/IF |
Use a concentration of 20 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 25 kDa. |
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ICC/IF
Use a concentration of 20 µg/ml. |
ターゲット情報
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機能
Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter (By similarity). Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear. -
組織特異性
Expressed in omental and subcutaneous adipose tissue (at protein level). -
関連疾患
In omental and subcutaneous adipose tissue of obese patients matched for BMI, expression levels correlate with insulin sensitivity. Expression is increased 5-6 fold in the group of patients with high insulin sensitivity, compared to the insulin-resistant group. This observation is consistent with the idea that triglyceride storage in adipocytes plays an important role in sequestering triglycerides and fatty acids away from the circulation and peripheral tissues, thus enhancing insulin sensitivity in liver and muscle. -
配列類似性
Contains 1 CIDE-N domain. -
細胞内局在
Lipid droplet. Nucleus. Enriched at lipid droplet contact sites. Has been shown to localize to mitonchondria, where it could interact with UCP1 and hence inhibit UCP1 uncoupling activity (By similarity). These data could not be confirmed (PubMed:18509062). - Information by UniProt
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参照データベース
- Entrez Gene: 12683 Mouse
- SwissProt: O70302 Mouse
- Unigene: 449 Mouse
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別名
- Cell death activator CIDE A antibody
- Cell death activator CIDE-A antibody
- Cell Death Inducing DFFA Like Effector A antibody
see all
画像
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Western blot - Anti-CIDE A antibody (ab8402)Anti-CIDE A antibody (ab8402) at 1/500 dilution + Murine heart tissue lysate
Predicted band size: 25 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-CIDE A antibody (ab8402)Immunofluorescence of CIDE-A in Mouse Heart cells using ab8402 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab8402 は 9 報の論文で使用されています。
- Zhou YP et al. Adenosine A2A receptor activation prevents DOCA-salt induced hypertensive cardiac remodeling via iBAT. Biochem Biophys Res Commun N/A:N/A (2020). PubMed: 32087969
- Liu W et al. Stat5a promotes brown adipocyte differentiation and thermogenic program through binding and transactivating the Kdm6a promoter. Cell Cycle 19:895-905 (2020). PubMed: 32207362
- Takahashi J et al. Interleukin-22 Directly Activates Myocardial STAT3 (Signal Transducer and Activator of Transcription-3) Signaling Pathway and Prevents Myocardial Ischemia Reperfusion Injury. J Am Heart Assoc 9:e014814 (2020). PubMed: 32301368
- Zhou J et al. Liraglutide induces beige fat development and promotes mitochondrial function in diet induced obesity mice partially through AMPK-SIRT-1-PGC1-a cell signaling pathway. Endocrine N/A:N/A (2018). PubMed: 30535743
- He Q et al. SREBP1c mediates the effect of acetaldehyde on Cidea expression in Alcoholic fatty liver Mice. Sci Rep 8:1200 (2018). PubMed: 29352167
- Ruan CC et al. A2A Receptor Activation Attenuates Hypertensive Cardiac Remodeling via Promoting Brown Adipose Tissue-Derived FGF21. Cell Metab 28:476-489.e5 (2018). PubMed: 30017353
- Zhang YY et al. Deletion of Macrophage Mineralocorticoid Receptor Protects Hepatic Steatosis and Insulin Resistance Through ERa/HGF/Met Pathway. Diabetes 66:1535-1547 (2017). PubMed: 28325853
- Binder AK et al. Expression of Human NSAID Activated Gene 1 in Mice Leads to Altered Mammary Gland Differentiation and Impaired Lactation. PLoS One 11:e0146518 (2016). PubMed: 26745373
- Carr SK et al. Maternal diet amplifies the hepatic aging trajectory of Cidea in male mice and leads to the development of fatty liver. FASEB J 28:2191-201 (2014). PubMed: 24481968