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  1. Link

    ciap1-antibody-ab2399.pdf

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Cell Biology Apoptosis Intracellular Survivin / IAPs
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Anti-cIAP1 抗体 (ab2399)

  • Datasheet
  • SDS
Submit a review Q&A (6)References (12)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

  • Western blot - Anti-cIAP1 antibody (ab2399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody (ab2399)

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関連製品

  • データシート
  • 文献 (12)
  • プロトコール
医薬用外毒物

製品の概要

  • 製品名

    Anti-cIAP1 antibody
    cIAP1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to cIAP1
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-P, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    The immunogen sequence of Rabbit polyclonal to cIAP1 (ab2399) was a peptide sequence flanking amino acid 530 of human cIAP1. (Peptide available as ab38272.)

  • ポジティブ・コントロール

    • WB: Jurkat cell lysate, PC12 cell lysate, rat kidney tissue lysate

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 30% Glycerol, 0.5% BSA, PBS, pH 7.2
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Survivin / IAPs
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • IAPs
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Metabolism
    • Types of disease
    • Cancer
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • IAPs
    • Cancer
    • Cell Death
    • Necroptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human cIAP1 protein (ab114410)

アプリケーション

Our Abpromise guarantee covers the use of ab2399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 69 kDa).

ターゲット情報

  • 機能

    Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
  • 組織特異性

    Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.
  • 配列類似性

    Belongs to the IAP family.
    Contains 3 BIR repeats.
    Contains 1 CARD domain.
    Contains 1 RING-type zinc finger.
  • 細胞内局在

    Cytoplasm.
  • Target information above from: UniProt accession Q13490 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 329 Human
    • Entrez Gene: 11797 Mouse
    • Entrez Gene: 60371 Rat
    • Omim: 601712 Human
    • SwissProt: Q13490 Human
    • SwissProt: Q62210 Mouse
    • Unigene: 696238 Human
    • Unigene: 335659 Mouse
    • Unigene: 205955 Rat
    see all
  • 別名

    • API 1 antibody
    • API1 antibody
    • Apoptosis inhibitor 1 antibody
    • Baculoviral IAP repeat containing 2 antibody
    • Baculoviral IAP repeat containing protein 2 antibody
    • Baculoviral IAP repeat-containing protein 2 antibody
    • BIRC 2 antibody
    • BIRC2 antibody
    • BIRC2_HUMAN antibody
    • C IAP1 antibody
    • C-IAP1 antibody
    • Cellular inhibitor of apoptosis 1 antibody
    • cellular inhibitor of apoptosis protein 1 antibody
    • cIAP 1 antibody
    • cIAP1 antibody
    • HIAP 2 antibody
    • HIAP-2 antibody
    • HIAP2 antibody
    • IAP 2 antibody
    • IAP homolog B antibody
    • IAP-2 antibody
    • IAP2 antibody
    • Inhibitor of apoptosis protein 2 antibody
    • MIHB antibody
    • NFR2 TRAF signalling complex protein antibody
    • RING finger protein 48 antibody
    • RNF 48 antibody
    • RNF48 antibody
    • TNFR2 TRAF signaling complex protein 2 antibody
    • TNFR2-TRAF-signaling complex protein 2 antibody
    see all

画像

  • Western blot - Anti-cIAP1 antibody (ab2399)
    Western blot - Anti-cIAP1 antibody (ab2399)
    Anti-cIAP1 antibody (ab2399) + Jurkat Cell lysate

    Predicted band size: 69 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody (ab2399)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody (ab2399)
    IHC image of ab2399 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2399, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

プロトコール

  • Western blot protocols
  • Immunohistochemistry protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献

    This product has been referenced in:

    • Cheng Z  et al. Lactobacillus raisesin vitroanticancer effect of geniposide in HSC-3 human oral squamous cell carcinoma cells. Exp Ther Med 14:4586-4594 (2017). Read more (PubMed: 29104666) »
    • Xu Q  et al. The cytoplasmic nuclear receptor RAR? controls RIP1 initiated cell death when cIAP activity is inhibited. Nat Commun 8:425 (2017). Read more (PubMed: 28871172) »
    See all 12 Publications for this product

    ab2399 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Question

    the monoclonal antibody we are using is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu28 of human c-IAP1 protein.

    The polyclonalantibody we are usingis produced by immunizing animals with a synthetic peptide corresponding to a region between the first two BIR domains of c-IAP1.

    Thank you for your help.

    Sincerely yours,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 17 2012

    Answer

    Thank you for your email.

    The peptide is from the sequence around amino acid 530 of human cIAP1. This sequence does not match with the immunogen sequence of polyclonal and monoclonal antibodies you have so this peptide ab38272 would be of no use.

    If you are interested in doing blocking experiment then ab38272 would block ab2399.

    I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Sep 17 2012

    Question

    I would like to test the specificity ofone of our anti-cIAP1 mAb by using your cIAP1 blocking peptide.

    I would therefore know if this peptide can be used as an immunogen for other antibodies than https://www.abcam.com/index.html?datasheet=2399.

    Thank you in advance for your kind reply.

    Sincerely yours,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 14 2012

    Answer

    Thank you for contacting us.

    Could you please explain what do you mean by other antibodies? The antibodies against the same peptide should be same however difference can be seen in specificity, purity etc.

    The peptide ab38272 is from a sequence flanking 530 of human cIAP1. We unfortunately don't know the exact sequence so this peptide may not able to block your antibody.

    Do you know the immunogen sequence of your antibody?

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Sep 14 2012

    Question

    samples: lysates of several human leucemic B-cells (BJAB, I-83, ...) ab2399 and ab32059: sees a band at 35 kDa, but not at 70 kDa as expected Ab: 1/50, 1/1000 overnight 4 degree regular PAGE system and Millipore's snap-system - same results secondary: OK block: 5% milk ab8421: sees high background and band at 55 kDa (however, band is not dominant), but all bands are of equal intensity Ab: 1/1000 and higher regular PAGE system and Millipore's snap-system - same results secondary: OK block: 5% milk

    Read More

    Abcam community

    Verified customer

    Asked on Nov 21 2011

    Answer

    Thank you for your phone call. I am very sorry to hear that these 3 antibodies are not providing satisfactory results. The details provided will enable us to investigate these 3 cases and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. As we discussed, I believe these 3 products should have given satisfactory results. It appears that you may have received either faulty vials, or the shipment was affected by temperature variations, customs or other unknown factors. I apologize very much for the inconvenience, but am pleased to let you know that I have arranged for the replacement shipment as we discussed. Thank you for your understanding. I wish you good luck with your research! Please let me know if there is anything else I can help you with.

    Read More

    Abcam Scientific Support

    Answered on Nov 21 2011

    Question

    I know the datasheet lists the immunogen as peptide ab38272, but I was wondering if it were possible to find out the exact sequence of the peptide used as the immunogen. I understand this isn't always possible, but please let me know if it is. Than

    Read More

    Abcam community

    Verified customer

    Asked on Oct 11 2011

    Answer

    Thank you for your inquiry. We source this antibody from an outside laboratory, and unfortunately we do not have access to the exact sequence used for the immunogen. If you plan to use the antibody on a fragment of the protein, we may be able to get more specific information of whether this antibody would bind to your fragment, but unfortunately we don't have access to any further information than what is on the datasheet. I apologize that I cannot be more help

    Read More

    Abcam Scientific Support

    Answered on Oct 11 2011

    Question

    We would like to know which region of cIAP1 does the antibody recognize (i.e. immunogen). Thanks!

    Read More

    Abcam community

    Verified customer

    Asked on Apr 27 2006

    Answer

    Thank you for your enquiry. The immunogen sequence of Rabbit polyclonal to cIAP1 (ab2399) was a peptide sequence flanking amino acid 530 of human cIAP1. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on May 02 2006

    Question

    i want to use it for immunohistochemistry, and have no idea whether it will work or not, does anybody used to try it on IHC?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 07 2004

    Answer

    Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

    Read More

    Abcam Scientific Support

    Answered on Oct 07 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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