Chymotrypsin Assay Kit (Fluorometric) (ab234051)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Tissue Lysate
製品の概要
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製品名
Chymotrypsin Assay Kit (Fluorometric) -
検出方法
Fluorescent -
サンプルの種類
Cell Lysate, Tissue Lysate -
種交差性
交差種: Mammals, Other species -
製品の概要
Chymotrypsin Assay Kit (Fluorometric) (ab234051) uses a synthetic fluorogenic substrate, enabling kinetic measurement of chymotrypsin activity in cell and tissue lysates. A chymotrypsin activator cleaves chymotrypsinogen to form active chymotrypsin, which then hydrolyzes the non-fluorescent substrate to release a stable fluorophore. The kit includes a selective chymotrypsin inhibitor that can be used to measure specific chymotrypsin activity in samples containing non-specific proteases and endopeptidases that may also metabolize the substrate. The assay can detect as low as 0.01 mU of Chymotrypsin.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K352 Chymotrypsin Activity Assay Kit (Fluorometric). K352-100 is the same size as the 100 test size of ab234051.
Chymotrypsin (EC 3.4.21.1) is a key serine protease involved in dietary protein digestion in mammals. It is primarily produced by the pancreas, but may be expressed in other tissues, including spleen and liver. In the pancreas, chymotrypsin is initially expressed as the inactive proenzyme chymotrypsinogen, which is cleaved by other proteases to chymotrypsin, its active form. Chymotrypsin specifically cleaves peptide bonds at the C-terminal end of bulky hydrophobic or aromatic amino acids (such as tyrosine, tryptophan or phenylalanine).
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests AMC Standard 1 x 100µl Assay Buffer I 1 x 25ml Chymotrypsin Activator 1 vial Chymotrypsin Inhibitor I 1 x 80µl Chymotrypsin Positive Control 1 vial Chymotrypsin Substrate 1 x 200µl -
研究分野
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機能
Has chymotrypsin-type protease activity and hypocalcemic activity. -
組織特異性
Pancreas. -
関連疾患
Variations in CTRC influence susceptibility to chronic pancreatitis [MIM:167800]. Chronic pancreatitis is a persistent inflammatory disorder characterized by permanent destruction of the pancreatic parenchyma. -
配列類似性
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
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別名
- Caldecrin
- Chymotrypsin C
- Chymotrypsin-C
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab234051 は 1 報の論文で使用されています。
- Huang H et al. Transgenic Expression of PRSS1R122H Sensitizes Mice to Pancreatitis. Gastroenterology 158:1072-1082.e7 (2020). PubMed: 31419436