Anti-CHX10 抗体 (ab16141)
Key features and details
- Sheep polyclonal to CHX10
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-CHX10 antibody
CHX10 一次抗体 製品一覧 -
製品の詳細
Sheep polyclonal to CHX10 -
由来種
Sheep -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Recombinant fragment corresponding to Human CHX10 aa 264-361 (C terminal).
Sequence:EAAAEKPEGERQALPKLDKMEQDERGPDAQAAISQEELRENSIAVLRAKA QEHSTKVLGTVSGPDSLARSTEKPEEEEAMDEDRPAERLSPPQLEDMA
Database link: P58304 -
特記事項
Chx10 is a 46kDa homeodomain protein of the paired-like class that is essential for development of the mammalian eye. Mutations in Chx10 cause microphthalmia, a cause of congenital blindness in humans, and the ocular retardation (or) phenotype in mice. In the developing mouse retina Chx10 is expressed in retinal progenitors, while in the mature retina, Chx10 expression becomes restricted to bipolar neurons. Concurrent with these expression patterns, the Chx10-/- (or) retina is thin due to a defect in proliferation of retinal progenitors, and lacks bipolar neurons. Chx10 is also expressed in the developing brainstem, thalamus, and spinal cord.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Ammonium Sulphate Precipitation -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
ChIP Related Products
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16141の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 46 kDa.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 46 kDa. |
ターゲット情報
-
機能
Plays a significant role in the specification and morphogenesis of the sensory retina. May also participate in the development of the cells of the inner nuclear layer, particularly bipolar cells. -
組織特異性
Abundantly expressed in retinal neuroblasts during eye development and in the inner nuclear layer of the adult retina. Within this layer, expression is stronger in the outer margin where bipolar cells predominate. -
関連疾患
Defects in VSX2 are the cause of microphthalmia isolated type 2 (MCOP2) [MIM:610093]; also known as isolated clinical anophthalmia. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataractand other abnormalities like cataract may also be present.
Defects in VSX2 are the cause of microphthalmia with cataracts and iris abnormalities (MCOPCTI) [MIM:610092].
Defects in VSX2 are the cause of microphthalmia isolated with coloboma type 3 (MCOPCB3) [MIM:610092]; also known as isolated colobomatous microphthalmia 3. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). -
配列類似性
Belongs to the paired homeobox family.
Contains 1 CVC domain.
Contains 1 homeobox DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 12677 Mouse
- Entrez Gene: 171360 Rat
- SwissProt: Q61412 Mouse
- Unigene: 4405 Mouse
-
別名
- C elegans ceh 10 homeo domain containing homolog antibody
- Ceh 10 homeo domain containing homolog (C. elegans) antibody
- Ceh 10 homeo domain containing homolog antibody
see all
画像
-
Western blot - Anti-CHX10 antibody (ab16141)
Western blot analysis using ab16141 at 1 µg/ml on rat liver (A), rat retina tissue lysate (B), mouse liver (C) and mouse retina tissue lysate (D).
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (18)
ab16141 は 18 報の論文で使用されています。
- Sheng W et al. Immunohistological Localization of Mel1a Melatonin Receptor in Pigeon Retina. Nat Sci Sleep 13:113-121 (2021). PubMed: 33574722
- Schnerwitzki D et al. Wt1 Positive dB4 Neurons in the Hindbrain Are Crucial for Respiration. Front Neurosci 14:529487 (2020). PubMed: 33328840
- Lindner M et al. Expression and Localization of Kcne2 in the Vertebrate Retina. Invest Ophthalmol Vis Sci 61:33 (2020). PubMed: 32191288
- Faustino Martins JM et al. Self-Organizing 3D Human Trunk Neuromuscular Organoids. Cell Stem Cell 26:172-186.e6 (2020). PubMed: 31956040
- Cui LJ et al. nGnG Amacrine Cells and Brn3b-negative M1 ipRGCs are Specifically Labeled in the ChAT-ChR2-EYFP Mouse. Invest Ophthalmol Vis Sci 61:14 (2020). PubMed: 32049344