Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Hippocampal neuron culture)
Specification
Hippocampal neuron culture
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 5%
Other product details
Concentration
2 µg/ml
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse IgG2b Alexa Fluor 568
Host species: Goat
Clonality: Polyclonal
Host species: Goat
Clonality: Polyclonal
Additional data
Additional Notes
Confocal image: ab20245 in red and nuclear counterstain in blue. No immunoreactivity was seen under these conditions when ab20245 was used at 0.7ug/ml. Patchy immunoreactivity was seen at 1ug/ml. Little cytosolic immunoreactivity.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Randal Moldrich
Verified customer
投稿 May 17 2006