Anti-Chromogranin A 抗体 (ab45179)
Key features and details
- Rabbit polyclonal to Chromogranin A
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Chromogranin A antibody
Chromogranin A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Chromogranin A -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Dog, Pig, Monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: PC-12 and SH-SY5Y cell lysates. IHC-P: Human pancreatic tissue and adenocarcinoma of pancreas tissue. ICC/IF: Panc-1 and SHSY5Y cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45179の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (8) |
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF |
Use a concentration of 5 µg/ml.
For unpurified use at 1/50 - 1/100. |
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WB |
1/2000. Detects a band of approximately 86 kDa.
For unpurified use at 1/10000. |
特記事項 |
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IHC-P
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 5 µg/ml. For unpurified use at 1/50 - 1/100. |
WB
1/2000. Detects a band of approximately 86 kDa. For unpurified use at 1/10000. |
ターゲット情報
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機能
Pancreastatin strongly inhibits glucose induced insulin release from the pancreas. -
配列類似性
Belongs to the chromogranin/secretogranin protein family. -
翻訳後修飾
Sulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans. -
細胞内局在
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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参照データベース
- Entrez Gene: 1113 Human
- Entrez Gene: 12652 Mouse
- Entrez Gene: 397540 Pig
- Entrez Gene: 24258 Rat
- Omim: 118910 Human
- SwissProt: P10645 Human
- SwissProt: P26339 Mouse
- SwissProt: P04404 Pig
see all -
製品の状態
According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa. -
別名
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
see all
画像
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Unpurified ab45179 staining Chromogranin A in pig small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/750 in blocking buffer) for 2 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
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Anti-Chromogranin A antibody (ab45179) at 1/5000 dilution (purified) + SH-SY5Y cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Observed band size: 86 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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ab45179 staining Chromogranin A in SHSY5Y cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab45179 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adenocarcinoma of pancreas tissue labelling Chromogranin A with purified ab45179 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Anti-Chromogranin A antibody (ab45179) at 1/1000 dilution (purified) + PC-12 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Observed band size: 86 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse pancreas tissue labelling Chromogranin A with purified ab45179 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Anti-Chromogranin A antibody (ab45179) at 1/10000 dilution (unpurified) + PC-12 cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Observed band size: 86 kDa why is the actual band size different from the predicted? -
Chromogranin A staining on monkey pancreas sections. The sections were subjected to heat mediated epitope retrieval using citric acid (pH 6) and blocked using 1% BSA for 10 mins at 21°C. Unpurifeid ab45179 was used at a dilution of 1/750 using TBS/BSA/Azide for 2 hours at 21°C. (Red arrowheads indicate extra Islet positive cells).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (26)
ab45179 は 26 報の論文で使用されています。
- Wang M et al. The developmental changes in intestinal epithelial cell proliferation, differentiation, and shedding in weaning piglets. Anim Nutr 9:214-222 (2022). PubMed: 35600553
- Perdigoto AL et al. Immune cells and their inflammatory mediators modify β cells and cause checkpoint inhibitor-induced diabetes. JCI Insight 7:N/A (2022). PubMed: 35925682
- Chen X et al. Androgen deprivation restores ARHGEF2 to promote neuroendocrine differentiation of prostate cancer. Cell Death Dis 13:927 (2022). PubMed: 36335093
- Engevik MA et al. Human-Derived Bifidobacterium dentium Modulates the Mammalian Serotonergic System and Gut-Brain Axis. Cell Mol Gastroenterol Hepatol 11:221-248 (2021). PubMed: 32795610
- Kasashima H et al. Stromal SOX2 Upregulation Promotes Tumorigenesis through the Generation of a SFRP1/2-Expressing Cancer-Associated Fibroblast Population. Dev Cell 56:95-110.e10 (2021). PubMed: 33207226