Anti-Chromogranin A 抗体 (ab15160)
製品の概要
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製品名
Anti-Chromogranin A antibody
Chromogranin A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Chromogranin A -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Fr, WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human, Monkey -
免疫原
Recombinant fragment within Human Chromogranin A aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P10645 -
ポジティブ･コントロール
- Pancreas
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab15160 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. PubMed: 21383188 | |
| WB | 1/100. | |
| ICC/IF | 1/100. | |
| IHC-P | 1/400. |
ターゲット情報
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機能
Pancreastatin strongly inhibits glucose induced insulin release from the pancreas. -
配列類似性
Belongs to the chromogranin/secretogranin protein family. -
翻訳後修飾
Sulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans. -
細胞内局在
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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参照データベース
- Entrez Gene: 1113 Human
- Entrez Gene: 12652 Mouse
- Omim: 118910 Human
- SwissProt: P10645 Human
- SwissProt: P26339 Mouse
- Unigene: 150793 Human
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製品の状態
According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa. -
別名
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody (ab15160)This image is courtesy of an anonymous Abreviewab15160 at 1/250 staining human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed before incubation with the antibody for 30 minutes at 25°C. An HRP conjugated rabbit polyclonal was used as the secondary.
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Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin A antibody (ab15160)This image is a courtesy of Anonymous Abreviewab15160 staining Chromogranin A in mouse gastric gland cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton ×100 and blocking with 10% serum for 45min at 200C was performed. Samples were incubated with primary antibody (1/100) for 18 hours at 4°C. A Texas Red ®-conjugated goat polyclonal to rabbit IgG was used at dilution 1/200 as secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody (ab15160)This image is courtesy of an anonymous Abreviewab15160 (1/100) staining Chromogranin A in paraffin-embbeded mouse pancreas tissue sections. Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in citrate buffer pH 6.0 and blocking (10 minutes, 1.5% serum). An HRP-conjugated goat polyclonal was used as the secondary. For further experimental details please refer to abreview. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody (ab15160)This image is a courtesy of Anonymous Abreviewab15160 staining Chromogranin A in mouse colon tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formalin fixation before heat mediated antigen retrieval with 10mM citric acid buffer and then the sample was blocked for 45 minutes at 20°C. The primary antibody (1/100) was incubated with sample in 10% serum for 18 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution 1/200 as secondary antibody.
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Western blot - Anti-Chromogranin A antibody (ab15160)Chromogranin A is easily fragmented and both bands observed in the Western Blot above are believed to correspond to Chromogranin A.
プロトコール
参考文献
This product has been referenced in:
- Wong C et al. Two well-differentiated pancreatic neuroendocrine tumor mouse models. Cell Death Differ 27:269-283 (2020). Read more (PubMed: 31160716) »
- Coquenlorge S et al. GLI2 Modulated by SUFU and SPOP Induces Intestinal Stem Cell Niche Signals in Development and Tumorigenesis. Cell Rep 27:3006-3018.e4 (2019). Read more (PubMed: 31167144) »
