製品名Chromatin Extraction Kit
Chromatin Extraction キット 製品一覧
サンプルの種類Tissue, Adherent cells, Suspension cells
Chromatin Extraction Kit (ab117152) is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.
保存方法Please refer to protocols.
内容 100 tests 1000X Protease Inhibitor Cocktail 1 x 110µl 10X Lysis Buffer 1 x 11ml Chromatin Buffer 1 x 11ml Extraction Buffer 1 x 11ml
ChIP analysis of RNA polymerase II enriched in GAPDH and MLH1 promoters with chromatin extract prepared from formaldehyde fixed colon cancer cells (2x105) using ab117152.
Chromatin was extracted from 3x106 mouse neuroblastoma cells, fixed for 10min at RT in 0.1% formaldehyde. Cells were sonicated before last centrifugation, and following centrifugation, cells were treated with proteinase K & RNAse A.
Chromatin yield was 4 µg chromatin/106 cells.Lane 1: 1kb DNA ladder Lane 2: 300 - 650bp DNA fragments
Image courtesy of anonymous Abreview
This product has been referenced in:
- Zhu B et al. The protective role of DOT1L in UV-induced melanomagenesis. Nat Commun 9:259 (2018). Read more (PubMed: 29343685) »
- Lu R et al. COPS5 amplification and overexpression confers tamoxifen-resistance in ERa-positive breast cancer by degradation of NCoR. Nat Commun 7:12044 (2016). Functional Studies ; Human . Read more (PubMed: 27375289) »