Rabbit monoclonal [EPR13024(B)] to Choline Acetyltransferase
Mouse, Rat, Human
Synthetic peptide within Human Choline Acetyltransferase aa 700 to the C-terminus. The exact sequence is proprietary.
Database link: P28329 (Peptide available as ab203107)
SH-SY5Y and Human fetal brain lysate. SH-SY5Y cells.
A trial size is available to purchase for this antibody.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/1000 - 1/10000. Predicted molecular weight: 82 kDa.
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Myasthenic syndrome, congenital, 6, presynaptic
Belongs to the carnitine/choline acetyltransferase family.
Information by UniProt
Acetyl CoA choline O acetyltransferase antibody
Acetyl CoA:choline O acetyltransferase antibody
Western blot - Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab181023)
All lanes :
Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab181023) at 1/5000 dilution
Lane 1 :
SH-SY5Y cell lysate
Lane 2 :
Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size:
Blocking/ Dilution buffer: 5% NFDM /TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab181023)
Immunofluorescent analysis of 4% paraformaldehyde fixed SH-SY5Y cells labeling Choline Acetyltransferase using ab181023 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/100 dilution.
Flow Cytometry - Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab181023)
Flow Cytometry analysis of SH-SY5Y cells labeling Choline Acetyltransferase using ab181023 at a 1/110 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
Immunoprecipitation - Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab181023)
Lysate from Human fetal brain (Lane 1) and negative control (Lane 2) were immunoprecipitated with ab181023 at a 1/70 dilution. A anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"