Anti-Choline Acetyltransferase 抗体 (ab18736)

Thank you very much for your interest in our products. The following antibodies are tested and guaranteed for IHC-P in rat. Unfortunately, we do not have blocking peptide for these antibodies in our catalogue. ab68779 Click here (or use the following: https://www.abcam.com/Choline-Acetyltransferase-antibody-ab68779.html). ab6168 Click here (or use the following: https://www.abcam.com/Choline-Acetyltransferase-antibody-ab6168.html). ab70219 Click here (or use the following: https://www.abcam.com/index.html?datasheet =70219). ab18736 Click here (or use the following: https://www.abcam.com/Choline-Acetyltransferase-antibody-ab18736.html). ab34419 Click here (or use the following: https://www.abcam.com/Choline-Acetyltransferase-antibody-ab34419.html). The following antibodies have a blocking peptide readily available, but are not tested and guaranteed for IHC-P. Therefore you might be eligible for our testing discount program if you would like to test one of these antibodies in IHC-P on rat samples. ab85609 with ab97420 Click here (or use the following: https://www.abcam.com/Choline-Acetyltransferase-antibody-C-terminal-ab85609.html). ab50412 with ab50440 Click here (or use the following: https://www.abcam.com/Choline-Acetyltransferase-antibody-ab50412.html). ab27484 with ab45678 Click here (or use the following: https://www.abcam.com/Choline-Acetyltransferase-antibody-ab27484.html). Please do not hesitate to contact me again with any further questions or if you consider testing one of the latter antibodies in IHC-P.

Thank you for your enquiry. Please find below the answers to your questions: Q1. was the fix of 4% formaldehyde containing 15% picric acid in 0.1M TBS,pH, 7.4 for 30 min done after the brain was extracted, and if so was the whole brain simply immersed in this solution for 30 mins or was there a perfusion step? If the brain was immersed - how do we know the fixative penetrated the entire brain in 30 mins? A. Animal is perfused with the fixative, then the brain is extracted and sectioned. Fresh brains can not be fixed properly in only 30 min. Q2. We find that we need to fix in 4% formaldehyde perfusion to extract spinal cord, what would be the effect of the perfusion? A. Perfusion with 4% PFA should not adversely affect the antigenicity of ChAT. We do that routinely without any problem. Q2Was staining done subsequently to rehydration? A. Please see the attached protocol on the datasheet. Q3. What were the ascending ethanol steps? A. Please see the attached protocol on the datasheet. Q4. Could this protocol be used on mounted sections (we have to mount spinal cord before fixation). If we do, should we apply the ethanol to frozen sections/slides, or should we let the slides reach room temp before starting to fix?? A. Please see the attached protocol for free floating section. Just note that you have to post-fix your sections if you are cutting your tissue freshly. Please do not hesitate to contact us if you require further assistance,