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  1. Link

    cholesterolcholesteryl-ester-assay-kit-detection-ab102515.pdf

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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cholesterol Metabolism
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Cholesterol/Cholesteryl Ester Assay Kit - Detection (ab102515)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (6)References (3)

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Functional Studies - Cholesterol/Cholesteryl Ester Detection Kit (ab102515)

    Key features and details

    • Assay type: Quantitative
    • Detection method: Colorimetric
    • Platform: Microplate reader
    • Assay time: 1 hr
    • Sample type: Cell Lysate, Tissue Lysate

    こちらの製品もご検討ください

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    関連製品

    製品の概要

    • 製品名

      Cholesterol/Cholesteryl Ester Assay Kit - Detection
      Cholesterol/Cholesteryl Ester キット 製品一覧
    • 検出方法

      Colorimetric
    • サンプルの種類

      Cell Lysate, Tissue Lysate
    • アッセイタイプ

      Quantitative
    • 全工程の試験時間

      1h 00m
    • 製品の概要

      Cholesterol/Cholesteryl Ester Assay Kit ab102515 uses a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both using a colorimetric method.


      This assay kit uses an alternative method compared to our most popular Cholesterol/ Cholesteryl Ester Assay Kit ab65359 (which relies on a cholesterol oxidase based method).


      In this assay kit, free cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at 450 nm.


      The assay can detect free or total cholesterol depending upon whether cholesterol esterase is used to hydrolyze cholesterol esters present.


      Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters).

    • 特記事項

      This product is manufactured by BioVision, an Abcam company and was previously called K623 Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II. K623-100 is the same size as the 100 test size of ab102515.

      Cholesterol is an essential molecule in all animal life. It has been involved in both normal development and diseases.

    • 試験プラットフォーム

      Microplate reader

    製品の特性

    • 保存方法

      Store at -20°C. Please refer to protocols.
    • 内容 ラベル 100 tests
      Cholesterol Assay Buffer WM 1 x 25ml
      Cholesterol Standard Yellow 1 x 100µl
      Enzyme Mix (lyophilized) Green 1 vial
      Esterase (lyophilized) Blue 1 vial
      Substrate Mix (lyophilized) Red 1 vial
    • 研究分野

      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipid Metabolism
      • Cholesterol Metabolism
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Cardiovascular
      • Atherosclerosis
      • Lipoprotein metabolism
      • Kits/ Lysates/ Other
      • Kits
      • Cell Metabolism Kits
      • Lipid Metabolism Kits
      • Cholesterol
      • Kits/ Lysates/ Other
      • Kits
      • Cell Metabolism Kits
      • Lipid Metabolism Kits
      • Obesity
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Cholesterol Metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipoprotein metabolism
    • 関連性

      Cholesterol is an essential molecule in all animal life. It is involved in both normal development and diseases. A high level of serum cholesterol is an indicator for diseases such as heart disease. Cholesteryl ester is an ester of cholesterol. The ester bond is formed between the carboxylate group of a fatty acid and the hydroxyl group of cholesterol.

    関連製品

    • Related Products

      • Cholesterol Assay Kit (Cell-Based) (ab133116)
      • Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation (ab65359)

    画像

    • Functional Studies - Cholesterol/Cholesteryl Ester Detection Kit (ab102515)
      Functional Studies - Cholesterol/Cholesteryl Ester Detection Kit (ab102515)
      Cholesterol Standard Curve: Cholesterol/Cholesteryl Ester was quantified according to the kit instructions. Background from the 0 standard reading (without cholesterol) has been subtracted from all readings.

    プロトコール

    • Protocol Booklet

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (3)

    ab102515 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab102515 は 3 報の論文で使用されています。

    • Ali A  et al. CAV1 - GLUT3 signaling is important for cellular energy and can be targeted by Atorvastatin in Non-Small Cell Lung Cancer. Theranostics 9:6157-6174 (2019). PubMed: 31534543
    • Liang X  et al. Organic cation transporter 1 (OCT1) modulates multiple cardiometabolic traits through effects on hepatic thiamine content. PLoS Biol 16:e2002907 (2018). PubMed: 29659562
    • Li X  et al. Fatostatin displays high antitumor activity in prostate cancer by blocking SREBP-regulated metabolic pathways and androgen receptor signaling. Mol Cancer Ther 13:855-66 (2014). PubMed: 24493696

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Question

    Dear
    Thank you for taking time to look at my question – will order a couple of kits and try it out.

    Kind regards

    Read More

    Abcam community

    Verified customer

    Asked on Aug 09 2012

    Answer

    I'm glad you found the information of help.

    I'd be very interested to know how you get on with the kit if you would mind letting me know. If you need any help with the kit please do not hesitate to contact us again.

    Until then, I wish you all the best with your research.

    Read More

    Abcam Scientific Support

    Answered on Aug 09 2012

    Question

    Phone call requesting information on it the Cholesterol/Cholesteryl Ester Quantitation Kit can be used with egg yolk samples.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 08 2012

    Answer

    Thank you for contacting us yesterday.

    I now have some further information to share with you in regards to the Cholesterol/Cholesteryl Ester Quantitation Kit and Cholesterol/Cholesteryl Ester Detection Kit (ab65359 and ab102515 respectively).

    As discussed over the phone, I have sought further information in regards to these kits and their suitability with egg samples. As mentioned on our product details page, this kit has as yet onlybeen tested for compatibility with mammalian samples. We have not tested egg yolk with either of thesekits. However, the source of this kit feels that there is no reason why it would not work. It would probably require some optimisation on your part to get the best results.

    I looked at the publications we have on file for this kit and could not find any pertaining to egg yolk samples. It does not mean that it has not been done yet, just not published prominently. I would still suggest it should be suitable. However, we would not be able to guarantee it.

    I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

    Read More

    Abcam Scientific Support

    Answered on Aug 08 2012

    Question

    I will be sending in an order today for abcam's cholesterols kit (ab102515 LDL/HDL/VLDL) urgently, but I have a question: For cholesterol determination in tissue samples it is required to extract lipids and after centrifugation air dry at 50 degrees and then under vacuum for a further 50min. If I dont have a vacuum, what can I do with the sample to extract the remaining buffer? Air dry it at 50 degrees for longer?




    Many thanks

    Read More

    Abcam community

    Verified customer

    Asked on Aug 02 2012

    Answer

    Thank you for your inquiry.

    I can confirm that without the help of vacuum the drying process will be much longer.

    But it is possible to do so by prolonging the air dry period at 50C.

    Since this has not be experimentally tested by us, the length of the air dry incubation has to be determined by your customer.

    I hope this information is helpful and wish your customer good luck with their experiments and their presentation.

    Read More

    Abcam Scientific Support

    Answered on Aug 02 2012

    Question

    Hello, We have order the Cholesterol/Cholesteryl Ester Kit, and were wondering if the kit is useble on mouse bile samples. Thanks!

    Read More

    Abcam community

    Verified customer

    Asked on May 31 2012

    Answer

    Thank you for contacting us.

    Yes, the kit is suitable for assay of mouse bile.

    Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on May 31 2012

    Question

    Dear Abcam I would like to investigate the levels of Cholesterol and cholesterol esters in cell lysates or homogenated tissue. I see that you have two kits for this purpose. My question is: Is it possible to measure directly on cell lysate or does it require purification of lipids? And if it is possible to measure without purification, what are the conditions?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 26 2012

    Answer

    Thank you for contacting us.

    Indeed, for thequantification of cholesterol and cholesterol esters with have the kits ab65359and ab102515 available.ab65359uses both colorimetric and fluorometric detection, whereas ab102515 uses only colorimetric detection, but this assay can tolerate interferences from various samples significantly.

    For best results we recommendextraction of lipids following theprotocol:

    For cells or tissue samples, 106 cells or 10 mg tissue can be extracted with 200µl of chloroform: Isopropanol:NP40 (7:11:0.1) in a microhomogenizer. Centrifuge the extract for 5-10 minutes at 15,000xg. Transfer all of the liquid (organic phase), avoiding the pellet, to a new tube and air dry at 50ºC to remove chloroform. Put the samples under vacuum for 30 minutes to remove trace organic solvent. Dissolve dried lipids with 200µl of Cholesterol Assay Buffer by sonicating or vortexing until homogeneous (it is all right if the solution becomes cloudy). The extraction procedure can be scaled up if larger amounts of sample are required. Use 1-50µl of extracted sample per assay adjusting the volume to 50µl/well with Cholesterol Assay Buffer. For unknown samples it is recommended that different amounts are tested to ensure that readings are within the linear portion of the standard curve.

    Unfortunately, we have not tested either kit without purification, so cannot make any further recommendations.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Apr 26 2012

    Question

    I have many samples to prepare and I'd like to measure them all together. What is a good stopping point during this protocol? Also, the protocol states "For cells or tissue samples, extract 106 cells or 10 mg tissue with 200 µl CHCl3:IPA:NP-40 (7:11:0.1) in a microhomogenizer." -->After adding 200 µl CHCl3:IPA:NP-40 to the cells, how should the cells be homegenized before centrifuging the extract for 5 min at 15,000xg?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 18 2011

    Answer

    1) A good stopping point would be after the samples are dried "(store at -20 °C until ready to proceed): Transfer the liquid phase to a new tube and air dry at 50°C then place samples under vacuum for 30 min to remove any remaining solvent." 2) Usually one does some 30-50 passes with a Dounce homogenizer- or you could try gently pipetting up and down (NO VORTEXING as this creates bubbles); or some sonication on ice if you do not have a homogenizer. Check a few microliters under the microscope to confirm you have good lysis of cells (if that is what you are working with). I hope this is helpful. Please contact us again if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Aug 18 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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