Cholesterol/Cholesteryl Ester Assay Kit - Detection (ab102515)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell Lysate, Tissue Lysate
製品の概要
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製品名
Cholesterol/Cholesteryl Ester Assay Kit - Detection
Cholesterol/Cholesteryl Ester キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell Lysate, Tissue Lysate -
アッセイタイプ
Quantitative -
全工程の試験時間
1h 00m -
製品の概要
Cholesterol/Cholesteryl Ester Assay Kit ab102515 uses a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both using a colorimetric method.
This assay kit uses an alternative method compared to our most popular Cholesterol/ Cholesteryl Ester Assay Kit ab65359 (which relies on a cholesterol oxidase based method).
In this assay kit, free cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at 450 nm.
The assay can detect free or total cholesterol depending upon whether cholesterol esterase is used to hydrolyze cholesterol esters present.
Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters).
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K623 Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II. K623-100 is the same size as the 100 test size of ab102515.
Cholesterol is an essential molecule in all animal life. It has been involved in both normal development and diseases.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 100 tests Cholesterol Assay Buffer WM 1 x 25ml Cholesterol Standard Yellow 1 x 100µl Enzyme Mix (lyophilized) Green 1 vial Esterase (lyophilized) Blue 1 vial Substrate Mix (lyophilized) Red 1 vial -
研究分野
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関連性
Cholesterol is an essential molecule in all animal life. It is involved in both normal development and diseases. A high level of serum cholesterol is an indicator for diseases such as heart disease. Cholesteryl ester is an ester of cholesterol. The ester bond is formed between the carboxylate group of a fatty acid and the hydroxyl group of cholesterol.
関連製品
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab102515 は 3 報の論文で使用されています。
- Ali A et al. CAV1 - GLUT3 signaling is important for cellular energy and can be targeted by Atorvastatin in Non-Small Cell Lung Cancer. Theranostics 9:6157-6174 (2019). PubMed: 31534543
- Liang X et al. Organic cation transporter 1 (OCT1) modulates multiple cardiometabolic traits through effects on hepatic thiamine content. PLoS Biol 16:e2002907 (2018). PubMed: 29659562
- Li X et al. Fatostatin displays high antitumor activity in prostate cancer by blocking SREBP-regulated metabolic pathways and androgen receptor signaling. Mol Cancer Ther 13:855-66 (2014). PubMed: 24493696