CHN 1 overexpression 293T lysate (whole cell) (ab94168)
製品の概要
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製品名
CHN 1 overexpression 293T lysate (whole cell) -
特記事項
ab94168 is a 293T cell transfected lysate in which Human CHN 1 has been transiently over-expressed using a pCMV-CHN 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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アプリケーション
適用あり: WBmore details
製品の特性
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Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
バッファー
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究分野
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背景
Function: GTPase-activating protein for p21-rac and a phorbol ester receptor. May play an important role in neuronal signal-transduction mechanisms. Tissue specificity: In neurons in brain regions that are involved in learning and memory processes. Disease: Defects in CHN1 are the cause of Duane retraction syndrome type 2 (DURS2) [MIM:604356]. Duane retraction syndrome is a congenital eye movement disorder characterized by a failure of cranial nerve VI (the abducens nerve) to develop normally, resulting in restriction or absence of abduction, adduction, or both, and narrowing of the palpebral fissure and retraction of the globe on attempted adduction. Undiagnosed in children, it can lead to amblyopia, a permanent uncorrectable loss of vision. Similarity: Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 Rho-GAP domain. Contains 1 SH2 domain. Developmental stage: Increases in amount during brain development coincident with synaptogenesis.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94168の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent dilution.
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特記事項 |
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WB
Use at an assay dependent dilution. |
画像
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ab94168 at 15μg/lane on an SDS-PAGE gel.
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All lanes : Anti-CHN 1 antibody (ab54610) at 1/500 dilution
Lane 1 : CHN 1 293T Transfected Lysate - Positive Control (ab94168)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab94168 は論文での使用が確認できていません。