Anti-CHMP4B / CHMP4C 抗体 (ab272638)
Key features and details
- Rabbit polyclonal to CHMP4B / CHMP4C
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CHMP4B / CHMP4C antibody -
製品の詳細
Rabbit polyclonal to CHMP4B / CHMP4C -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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免疫原
Recombinant fragment corresponding to Human CHMP4B/ CHMP4C aa 1-100.
Database link: Q9H444 -
ポジティブ･コントロール
- WB: HEK-293T lysate. IHC-P: Human kidney tissue, small intestine tissue, liver tissue.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
| 特記事項 |
|---|
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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関連性
CHMP4B and CHMP4C belong to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 128866 Human
- Entrez Gene: 92421 Human
- Omim: 610897 Human
- Omim: 610899 Human
- SwissProt: Q96CF2 Human
- SwissProt: Q9H444 Human
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別名
- C20orf178 antibody
- Charged multivesicular body protein 4b antibody
- Charged multivesicular body protein 4c antibody
see all
画像
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Western blot - Anti-CHMP4B / CHMP4C antibody (ab272638)All lanes : Anti-CHMP4B / CHMP4C antibody (ab272638) at 0.4 µg/ml
Lane 1 : Control (vector only transfected HEK-293T lysate)
Lane 2 : CHMP4B over-expression lysate (mammalian HEK-293T cells) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHMP4B / CHMP4C antibody (ab272638)Paraffin-embedded formalin-fixed Human kidney tissue stained for CHMP4B / CHMP4C using ab272638 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHMP4B / CHMP4C antibody (ab272638)Paraffin-embedded formalin-fixed Human small intestine tissue stained for CHMP4B / CHMP4C using ab272638 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHMP4B / CHMP4C antibody (ab272638)Paraffin-embedded formalin-fixed Human liver tissue stained for CHMP4B / CHMP4C using ab272638 at 1/500 in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272638 は論文での使用が確認できていません。
