Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-CHMP2B 抗体 [EPR10807(B)] (ab157208)

Submit a review Submit a question
Western blot - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
  • Immunoprecipitation - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
  • Flow Cytometry - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
  • Immunocytochemistry - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
  • Western blot - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
  • Anti-CHMP2B antibody [EPR10807(B)] (ab157208)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10807(B)] to CHMP2B
  • Suitable for: ICC, Flow Cyt (Intra), WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-CHMP2B antibody [EPR10807(B)]
    CHMP2B 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10807(B)] to CHMP2B

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC, Flow Cyt (Intra), WB, IPmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Wild-type A549 whole cell lysate, Hela, 293T, MCF7 whole cell lysates; Mouse brain lysate, Rat brain lysate, Rat heart lysate ICC/IF: HepG2 cells; Flow cyt: 293T cells; IP: 293T whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab157208の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
1/250.

For unpurified use at 1/250 - 1/500 dilution.
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/10 - 1/100 dilution.
WB
1/1000. Predicted molecular weight: 24 kDa.

For unpurified use at 1/10000 - 1/10000 dilution.
IP
1/20.

For unpurified use at 1/10 - 1/100 dilution.
特記事項
ICC
1/250.

For unpurified use at 1/250 - 1/500 dilution.
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/10 - 1/100 dilution.
WB
1/1000. Predicted molecular weight: 24 kDa.

For unpurified use at 1/10000 - 1/10000 dilution.
IP
1/20.

For unpurified use at 1/10 - 1/100 dilution.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4.

  • 組織特異性

    Widely expressed. Expressed in brain, heart, skeletal muscle, spleen, kidney, liver, small intestine, pancreas, lung, placenta and leukocytes. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal chord, occipital lobe, frontal lobe, temporal lobe and putamen.

  • 関連疾患

    Defects in CHMP2B are the cause of frontotemporal dementia, chromosome 3-linked (FTD3) [MIM:600795]. FTD3 is characterized by an onset of dementia in the late 50's initially characterized by behavioral and personality changes including apathy, restlessness, disinhibition and hyperorality, progressing to stereotyped behaviors, non-fluent aphasia, mutism and dystonia, with a marked lack of insight. The brains of individuals with FTD3 have no distinctive neuropathological features. They show global cortical and central atrophy, but no beta-amyloid deposits.

  • 配列類似性

    Belongs to the SNF7 family.

  • ドメイン

    The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.

  • 細胞内局在

    Cytoplasm > cytosol. Late endosome membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ALS17 antibody
      • Charged multivesicular body protein 2b antibody
      • CHM2B_HUMAN antibody
      • CHMP family, member 2B antibody
      • CHMP2.5 antibody
      • CHMP2b antibody
      • Chromatin modifying protein 2b antibody
      • Chromatin-modifying protein 2b antibody
      • DMT1 antibody
      • hVps2-2 antibody
      • Vacuolar protein sorting 2, yeast, homolog of, B antibody
      • Vacuolar protein sorting 2-2 antibody
      • Vacuolar protein sorting-associated protein 2-2 antibody
      • VPS2 homolog B antibody
      • Vps2-2 antibody
      • VPS2B antibody
      see all

    画像

    • Western blot - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
      Western blot - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
      All lanes : Anti-CHMP2B antibody [EPR10807(B)] (ab157208) at 1/1000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
      Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
      Lane 4 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
      Lane 5 : Mouse brain lysate
      Lane 6 : Mouse heart lysate
      Lane 7 : Rat brain lysate
      Lane 8 : Rat heart lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Lanes 1-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
      Lane 8 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

      Predicted band size: 24 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?



      We are unsure about the nature of the 27kDa band. It may be isoform 2 of CHMP2B.

    • Immunoprecipitation - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
      Immunoprecipitation - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)

      Purified ab157208 at 1:20 dilution (0.7µg) immunoprecipitating CHMP2B in 293T whole cell lysate.


      Lane 1 (input): 293T (Human embryonic kidney epithelial cell) whole cell lysate 10µg.
      Lane 2 (+): ab157208 + 293T whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab157208 in 293T whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP)(ab131366) (1:1000 dilution) was used for Western blotting.


      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 30 kDa
      We are unsure about the nature of the 27kDa band. It may be isoform 2 of CHMP2B.

    • Flow Cytometry - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
      Flow Cytometry - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
      Flow Cytometry analysis of 293T (Human embryonic kidney epithelial cell) cells labelling CHMP2B with Purified ab157208 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    • Immunocytochemistry - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
      Immunocytochemistry - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
      Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling CHMP2B with Purified ab157208 at 1:250 dilution (0.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Western blot - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
      Western blot - Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
      All lanes : Anti-CHMP2B antibody [EPR10807(B)] (ab157208) at 1/1000 dilution

      Lane 1 : Wild-type A549 whole cell lysate
      Lane 2 : CHMP2B knockout A549 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : MCF7 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 24 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab157208 observed at 45 kDa. Red - loading control, ab8245, observed at 38 kDa.

      ab157208 was shown to specifically react with CHMP2B in wild-type A549 cells as signal was lost in CHMP2B knockout cells. Wild-type and CHMP2B knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab157208 and ab8245 (Mouse monoclonal [6C5] to GAPDH - Loading Control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Anti-CHMP2B antibody [EPR10807(B)] (ab157208)
      Anti-CHMP2B antibody [EPR10807(B)] (ab157208)

    参考文献 (0)

    ab157208 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab157208 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab157208.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com