Anti-CHMP2A/BC2 抗体 (ab67064)
Key features and details
- Mouse polyclonal to CHMP2A/BC2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CHMP2A/BC2 antibody -
製品の詳細
Mouse polyclonal to CHMP2A/BC2 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human CHMP2A/BC2 aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- Transfected 293T cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67064の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 25 kDa.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 25 kDa. |
ターゲット情報
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機能
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release. -
配列類似性
Belongs to the SNF7 family. -
ドメイン
The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components. -
細胞内局在
Late endosome membrane. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body. - Information by UniProt
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参照データベース
- Entrez Gene: 27243 Human
- Omim: 610893 Human
- SwissProt: O43633 Human
- Unigene: 12107 Human
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別名
- BC 2 antibody
- BC2 antibody
- Charged multivesicular body protein 2a antibody
see all
画像
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All lanes : Anti-CHMP2A/BC2 antibody (ab67064) at 1/500 dilution
Lane 1 : CHMP2A/BC2 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 25 kDa
Observed band size: ~28 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (1)
ab67064 は 1 報の論文で使用されています。
- Zhang Y et al. Proteomic Profiling of Purified Rabies Virus Particles. Virol Sin 35:143-155 (2020). PubMed: 31429011