Chloroquine diphosphate, apoptosis and autophagy inhibitor (ab142116)
Key features and details
- Antimalarial; apoptosis and autophagy inhibitor
- CAS Number: 50-63-5
- Purity: > 98%
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
Chloroquine diphosphate, apoptosis and autophagy inhibitor -
製品の詳細
Antimalarial; apoptosis and autophagy inhibitor -
生理活性の詳細
Antimalarial agent. Inhibits tumor cell growth and metastasis and induces apoptosis in vitro. Binds to Fe(II)-protoporphyrin IX (FP) to form FP-chloroquine complex resulting in cell lysis and parasite cell autodigestion.
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精製度
> 98% -
CAS 番号
50-63-5 -
構造式
製品の特性
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体系名
N4-(7-Chloroquinolin-4-yl)-N1,N1-diethylpentane-1,4-diamine diphosphate -
分子量
515.87 -
分子式
C18H26ClN3.2H3PO4 -
PubChem 登録番号
64927 -
保存方法
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in water to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
CCN(CC)CCCC(C)NC1=C2C=CC(=CC2=NC=C1)Cl.OP(=O)(O)O.OP(=O)(O)O -
由来
Synthetic
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研究分野
画像
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ab240635 staining SQSTM1 in wild-type Hap1 cells and SQSTM1 knockout Hap1 cells treated with chloroquine (ab142116, 50μM for 24 hrs). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab240635 at 1μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
2D chemical structure image of ab142116, Chloroquine diphosphate, apoptosis and autophagy inhibitor
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (13)
ab142116 は 13 報の論文で使用されています。
- Zhou L et al. Convergence of secretory, endosomal, and autophagic routes in trans-Golgi-associated lysosomes. J Cell Biol 222:N/A (2023). PubMed: 36239631
- Bhise N et al. Repurposing of artesunate, an antimalarial drug, as a potential inhibitor of hepatitis E virus. Arch Virol 168:147 (2023). PubMed: 37115342
- Frietze KK et al. Lipotoxicity reduces DDX58/Rig-1 expression and activity leading to impaired autophagy and cell death. Autophagy 18:142-160 (2022). PubMed: 33966599
- Alsamri H et al. Carnosol Induces p38-Mediated ER Stress Response and Autophagy in Human Breast Cancer Cells. Front Oncol 12:911615 (2022). PubMed: 35712465
- Alsamri H et al. Carnosol Is a Novel Inhibitor of p300 Acetyltransferase in Breast Cancer. Front Oncol 11:664403 (2021). PubMed: 34055630