製品名ChIP Kit (low cell number)
ChIP Kit (low cell number) (ab270814) contains everything you need for start-to-finish ChIP including all validated buffers and reagents for chromatin shearing, immunoprecipitation, and elution for exceptional ChIP-qPCR or ChIP-Seq results. In addition, positive control antibodies and negative control PCR primers are included for your convenience and assurance of result sensitivity and specificity.
Cells (10,000 cells per IP)
This kit protocol has been optimized for ChIP-seq on an Illumina® Next-Gen sequencer. The recommended amount of starting material for the Illumina® sample prep is 10-20 ng of IP’d DNA.
保存方法Please refer to protocols.
内容 16 tests Bead Wash Buffer BW1 1 x 13.6ml ChIP Buffer C1 1 x 4.5ml ChIP-seq grade antibody H3K4me3 1 x 8µg ChIP-seq grade GAPDH TSS primer pair 1 x 40µl ChIP-seq grade Myoglobin exon 2 primer pair 1 x 40µl Co-precipitant CP1 1 x 50µl Co-precipitant CP2 1 x 50µl Protein A-coated magnetic beads 1 x 180µl Elution Buffer E1 1 x 11ml Elution Buffer E2 1 x 410µl 1.25 M Glycine 1 x 4.5ml Lysis Buffer L1 1 x 1ml Precipitant P1 1 x 1ml Protease inhibitor cocktail 200x (PIC) 1 x 225µl Rabbit IgG 1 x 8µg Wash Buffer W1 1 x 4.5ml Wash Buffer W2 1 x 4.5ml Wash Buffer W3 1 x 4.5ml Wash Buffer W4 1 x 4.5ml