ChIP Kit (ab500)
Key features and details
- Sample type: Adherent cells, Suspension cells
製品の概要
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製品名
ChIP Kit
ChIP Kit キット 製品一覧 -
サンプルの種類
Adherent cells, Suspension cells -
製品の概要
ChIP kit ab500 provides a protocol and reagents for running ChIP assays including:
- cell lysis and chromatin extraction
- chromatin shearing and DNA fragment length analysis
- immunoprecipitation and DNA purification
DNA produced using the kit can be analyzed using qPCR.
The kit has been validated for ChIP assays with mammalian samples.
Chinese protocol available. See protocols section below.
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特記事項
This kit uses Protein A sepharose beads for antibody pulldown. See table below for Protein A and Protein G binding affinities with antibodies from commonly used species. For other species of antibody, consult the table in the protocol booklet.
Species raised in
Isotype Protein A binding affinity Protein G binding affinity Rabbit All isotypes +++ ++ Goat All isotypes - ++ Mouse
IgG1 + +++ IgG2a +++ +++ IgG2b ++ ++ IgG3 + + IgM Use anti-mouse IgM ChIP assay products and guides
Find more ChIP assay / chromatin immunoprecipitation resources and products, ChIP antibody products, and other ChIP assay kits and related reagents.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
アプリケーション
適用あり: ChIPmore details
適用なし: ChIP-seq
製品の特性
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保存方法
Please refer to protocols. -
内容 1 kit 1.25M Glycine 1 x 10ml Buffer A 1 x 10ml Buffer B 1 x 30ml Buffer C 1 x 30ml Buffer D 1 x 3ml ChIP Buffer 5X 1 x 84ml DNA Purifying Slurry 1 x 4ml ab1791 - H3 antibody 1 x 25µg PCR-Grade Water 1 x 10.2ml Protease Inhibitors 1 tablet Protein A Beads Unblocked 1 x 960µl Proteinase K 1 x 40µl -
研究分野
関連製品
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab500の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ChIP |
Use at an assay dependent concentration.
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特記事項 |
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ChIP
Use at an assay dependent concentration. |
画像
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Chromatin immunoprecipitation assay was performed to define the interaction of serum response factor (SRF) with the intragenic serum response element (SRE) regulatory motif in mouse using ab500 ChIP Kit.
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Chromatin immunoprecipitation using ab500 ChIP Kit and Histone H3 (tri methyl K9) antibody (ab8898). Chromatin was prepared from HeLa cells using the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. ChIP was performed with 2 µg of ab8898 (blue). No antibody was added to the beads control (yellow). Immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active/inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of transcribed region.
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Chromatin immunoprecipitation using ab500 ChIP Kit and Histone H3 (tri methyl K4) antibody (ab12209). Chromatin was prepared from HeLa cells using the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. ChIP was performed with 5 µg of ab12209 (blue). No antibody was added to the beads control (yellow). Immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active/inactive loci). Primers and probes are located in the first kb of the transcribed region.
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Chromatin immunoprecipitation using ab500 ChIP Kit and Histone H3 antibody (ab1791). Chromatin was prepared from HeLa cells using the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. ChIP was performed with 2 µg of ab1791 (blue). No antibody was added to the beads control (yellow). Immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active/inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (134)
ab500 は 134 報の論文で使用されています。
- Lago-Docampo M et al. Common Variation in EDN1 Regulatory Regions Highlights the Role of PPARγ as a Key Regulator of Endothelin in vitro. Front Cardiovasc Med 9:823133 (2022). PubMed: 35282351
- Zhang S et al. CTB-193M12.5 Promotes Hepatocellular Carcinoma Progression via Enhancing NSD1-Mediated WNT10B/Wnt/β-Catenin Signaling Activation. J Hepatocell Carcinoma 9:553-569 (2022). PubMed: 35698644
- Ji J et al. Lamin B2 contributes to the proliferation of bladder cancer cells via activating the expression of cell division cycle‑associated protein 3. Int J Mol Med 50:N/A (2022). PubMed: 35775376
- Liu M et al. p53 Binding Sites in Long Terminal Repeat 5Hs (LTR5Hs) of Human Endogenous Retrovirus K Family (HML-2 Subgroup) Play Important Roles in the Regulation of LTR5Hs Transcriptional Activity. Microbiol Spectr 10:e0048522 (2022). PubMed: 35867400
- Yang C & Liu H Both a hypoxia-inducible EYA3 and a histone acetyltransferase p300 function as coactivators of SIX5 to mediate tumorigenesis and cancer progression. Ann Transl Med 10:752 (2022). PubMed: 35957720