Chicken Anti-Mouse IgG H&L (FITC) (ab6810)

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Flow Cytometry - Chicken Anti-Mouse IgG H&L (FITC) (ab6810)

    Key features and details

    • Chicken Anti-Mouse IgG H&L (FITC)
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Host species: Chicken
    • Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA

    製品の概要

    • 製品名

      Chicken Anti-Mouse IgG H&L (FITC)
      IgG 二次抗体 製品一覧

    • 由来種

      Chicken

    • ターゲット生物種

      Mouse

    • アプリケーション

      適用あり: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details

    • 免疫原

      mouse whole molecule

    • 標識

      FITC. Ex: 493nm, Em: 528nm

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C.

    • バッファー

      Preservative: 0.01% Sodium azide
      Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption.

    • 特記事項（標識）

      Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG

    • ポリ/モノ

      ポリクローナル

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab6810の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent dilution.
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ELISA
    1/10000 - 1/50000.
    特記事項
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent dilution.
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ELISA
    1/10000 - 1/50000.

    画像

    • Flow Cytometry - Chicken Anti-Mouse IgG H&L (FITC) (ab6810)
      Flow Cytometry - Chicken Anti-Mouse IgG H&L (FITC) (ab6810)
      Ab6810 was used with the primary antibody ab2213 in Flow Cyt. See Abreview on ab2213.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (2)

    ab6810 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6810 は 2 報の論文で使用されています。

    • Arutyunyan I  et al. Role of VEGF-A in angiogenesis promoted by umbilical cord-derived mesenchymal stromal/stem cells: in vitro study. Stem Cell Res Ther 7:46 (2016). IF . PubMed: 27001300
    • Balaji Raghavendran HR  et al. A comparative study on in vitro osteogenic priming potential of electron spun scaffold PLLA/HA/Col, PLLA/HA, and PLLA/Col for tissue engineering application. PLoS One 9:e104389 (2014). ICC/IF . PubMed: 25140798

    レビューと Q&A

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    Question

    Hello, I know it says purified IgG, but has it been affinity purified so that it does not cross react with other species. The antibody will be mixed with donkey, goat and other secondary antibodies.

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    Answer

    All the information available for this product is stated on the datasheet. The datasheet reads "This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities". This antibody is therefore specific for mouse immunoglobulins.

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