Anti-CHD4 抗体 (ab72418)
Key features and details
- Rabbit polyclonal to CHD4
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-CHD4 antibody
CHD4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CHD4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to a region between residues 25 and 75 of human CHD4 (NP_001264.2)
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ポジティブ・コントロール
- WB: HeLa, Jurkat and HEK-293T cell lysates; IP: HeLa cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab72418 was affinity purified using an epitope specific to CHD4 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72418の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 218 kDa).
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IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 218 kDa). |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. -
配列類似性
Belongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 PHD-type zinc fingers. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase. - Information by UniProt
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参照データベース
- Entrez Gene: 1108 Human
- Omim: 603277 Human
- SwissProt: Q14839 Human
- Unigene: 162233 Human
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別名
- ATP dependent helicase CHD4 antibody
- ATP-dependent helicase CHD4 antibody
- CHD 4 antibody
see all
画像
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All lanes : Anti-CHD4 antibody (ab72418) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 218 kDa
Exposure time: 3 minutesLysates prepared using NETN lysis buffer.
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Detection of CHD4 by Western Blot of Immunprecipitate.
ab72418 at 0.1µg/ml staining CHD4 in HeLa whole cell lysate immunoprecipitated using ab72418 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (46)
ab72418 は 46 報の論文で使用されています。
- Zhong Y et al. Hierarchical regulation of the resting and activated T cell epigenome by major transcription factor families. Nat Immunol 23:122-134 (2022). PubMed: 34937932
- Jevtic Z et al. SMARCA5 interacts with NUP98-NSD1 oncofusion protein and sustains hematopoietic cells transformation. J Exp Clin Cancer Res 41:34 (2022). PubMed: 35073946
- Robbe ZL et al. CHD4 is recruited by GATA4 and NKX2-5 to repress noncardiac gene programs in the developing heart. Genes Dev 36:468-482 (2022). PubMed: 35450884
- de Castro RO et al. Mouse Chd4-NURD is required for neonatal spermatogonia survival and normal gonad development. Epigenetics Chromatin 15:16 (2022). PubMed: 35568926
- Schilders KAA et al. Identification of SOX2 Interacting Proteins in the Developing Mouse Lung With Potential Implications for Congenital Diaphragmatic Hernia. Front Pediatr 10:881287 (2022). PubMed: 35615634