Key features and details
- Chicken polyclonal to CGRP
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgY
CGRP 一次抗体 製品一覧
製品の詳細Chicken polyclonal to CGRP
アプリケーション適用あり: IHC-Pmore details
交差が予測される動物種: Mouse, Dog
- Trigeminal ganglion neurons
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
バッファーPreservative: 0.05% Sodium azide
Concentration information loading...
精製度Protein L purified
The Abpromise guarantee
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
関連性CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin.
細胞内局在Endoplasmic reticulum and Secreted
- Calcitonin/calcitonin related polypeptide alpha antibody
- Alpha type CGRP antibody
- Beta type CGRP antibody
Immunohistochemical analysis of expression of CGRP using ab92609.
A: the expression of CGRP is shown in the entire trigeminal ganglion obtained from an untreated adult rat.
B: at higher magnification (bar = 20µm), neuronal processes (arrows) expressing CGRP that extend from neuronal cell bodies in one cluster to another cluster are observed.
Protocol: ab92609 was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature.Blocking buffer was removed and slides were incubated for 18-24 hours at 4ºC with 1/100 ab92609. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were