Anti-cGMP 抗体 (ab836)

Submit a review Q&A (3)References (2)

Key features and details

  • Sheep polyclonal to cGMP
  • Suitable for: ELISA, RIA, Other
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-cGMP antibody
    cGMP 一次抗体 製品一覧

  • 製品の詳細

    Sheep polyclonal to cGMP

  • 由来種

    Sheep

  • 特異性

    This antibody reacts specifically with cyclic GMP.

  • アプリケーション

    適用あり: ELISA, RIA, Othermore details

  • 免疫原

    Succinylated cGMP conjugated to bovine serum albumin.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration. Able to detect and quantitate cGMP; 50% binding is achieved with a 1:6,400 dilution.
AP Use at an assay dependent concentration.
Other Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Cyclic guanosine monophosphate (cGMP) serves as a second messenger in a manner similar to that observed with cAMP. Peptide hormones, such as the natriuretic factors, activate receptors that are associated with membrane-bound guanylate cyclase (GC). Receptor activation of GC leads to the conversion of GTP to cGMP. Nitric oxide (NO) also stimulates cGMP production by activating soluble GC, perhaps by binding to the heme moiety of the enzyme. Similar to cAMP, cGMP mediates most of its intracellular effects through the activation of specific cGMP dependent protein kinases (PKG).

  • 別名

    • Cyclic GMP antibody
    • Cyclic guanosine monophosphate antibody
    • Guanosine 3 5 Cyclic Monophosphate antibody

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (2)

ab836 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab836 は 2 報の論文で使用されています。

  • Wu W  et al. Enhancing natriuretic peptide signaling in adipose tissue, but not in muscle, protects against diet-induced obesity and insulin resistance. Sci Signal 10:N/A (2017). PubMed: 28743802
  • Yu L  et al. A novel bioassay for the activity determination of therapeutic human brain natriuretic peptide (BNP). PLoS One 7:e49934 (2012). ELISA . PubMed: 23185490

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Question

I ordered this antibody and should have received 100 ul, but only received 20 ul.

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry that you did not receive the total volume purchased. As requested, I have issued a free of charge replacement.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

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Question

I am planning to purchase your anti-cGMP antibody for cGMP RIA. According to your website, it is not necessary to succinylate cGMP for the immune reaction. Does it mean that your antibody does not bind to succinylated cGMP? My iodine-labeled cGMP has succinyl group. If the antibody binds to succinylated cGMP, what buffer do you recommend me to use? I am currently using 90mM sodium acetate (pH 6.2). For RIA of cyclic nucleotides, I think a buffer of lower pH is commonly used.

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Answer

Thank you for contacting us. Here is the information I received from our source of this antibody: The anti-cyclic GMP antiserum does recognize succinylated cGMP. Unfortunately, I do not have the protocol used to generate these data. However, the small amount of information we do have indicates that the pH of the buffer used was 5.8. I am attaching the data that I received with this message. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Hello, When you use this antibody in immunoassays do you have to acetylate the samples to get good sensitivity since the immunogen was succinylated? Elizabeth Kenten

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Answer

For the development of this sheep antibody to cGMP, cGMP was succinylated in order to create a site to which BSA could be conjugated, thus creating the antigen that was used to immunize sheep. However, no treatment of samples is necessary in order for this antibody to recognize and bind to cGMP.

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