Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa and RPE-1)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris (MES Buffer))
Loading amount
25 µg
Specification
HeLa and RPE-1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: in 5% Milk
Dilution
1/2500
Secondary antibody
Dilution
1/20000
Name
Non-Abcam antibody was used: LICOR anti-rabbit IRDye680
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680RD
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680RD
Detection
Detection method
Near-infrared fluorescence
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 97 kDa Non-specific: 70, 55 (RPE-1 only) kDa
Additional data
Additional Notes
lysis buffer: PBS + 0.5% Triton X-100
lane 1: 25 ug Hela extract
lane 2: 25 ug RPE-1 extract
lane 3: 50 ug Hela extract
lane 4: 50 ug RPE-1 extract
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 10 2012