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    cellubrevin-antibody-ab5789.pdf

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Neuroscience Neurotransmission Secretory Vesicles Other
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Anti-Cellubrevin 抗体 (ab5789)

  • Datasheet
Reviews (1)Q&A (7)References (5)

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Western blot - Anti-Cellubrevin antibody (ab5789)
  • Western blot - Anti-Cellubrevin antibody (ab5789)
  • Western blot - Anti-Cellubrevin antibody (ab5789)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody (ab5789)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody (ab5789)

Key features and details

  • Rabbit polyclonal to Cellubrevin
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human, Recombinant fragment
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Cellubrevin antibody [EPR16866] (ab200657)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Cellubrevin antibody
    Cellubrevin 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Cellubrevin
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Rat, Human, Recombinant fragment
  • 免疫原

    Synthetic peptide corresponding to Mouse Cellubrevin aa 1-18.
    Sequence:

    MSTGVPSGSSAATGSNRR


    (Peptide available as ab5861)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ・コントロール

    • WB: HeLa cell extract (ab7898) ; Mouse Brain ; Rat Brain ICC/IF: NIH/3T3 (Mouse embryonic fibroblast cell line) ; Neuro-2a (Mouse neuroblastoma cell line)
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 一次抗体 備考

    The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer’s disease, all VAMP isoform levels appear to be significantly reduced.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Other
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • SNAPs & SNAREs
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Neuroscience
    • Processes

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Cellubrevin protein (ab86097)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab5789の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/500. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa).
ICC/IF
1/50.
特記事項
WB
1/500. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa).
ICC/IF
1/50.

ターゲット情報

  • 機能

    SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.
  • 配列類似性

    Belongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在

    Membrane. Cell junction > synapse > synaptosome.
  • Target information above from: UniProt accession Q15836 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 9341 Human
    • Entrez Gene: 22319 Mouse
    • Entrez Gene: 29528 Rat
    • Omim: 603657 Human
    • SwissProt: Q15836 Human
    • SwissProt: P63024 Mouse
    • SwissProt: P63025 Rat
    • Unigene: 66708 Human
    • Unigene: 273930 Mouse
    • Unigene: 219999 Rat
    see all
  • 別名

    • CEB antibody
    • Cellubrevin antibody
    • Synaptobrevin 3 antibody
    • Synaptobrevin-3 antibody
    • VAMP 3 antibody
    • VAMP-3 antibody
    • VAMP3 antibody
    • VAMP3_HUMAN antibody
    • Vesicle associated membrane protein 3 antibody
    • Vesicle-associated membrane protein 3 antibody
    see all

画像

  • Western blot - Anti-Cellubrevin antibody (ab5789)
    Western blot - Anti-Cellubrevin antibody (ab5789)
    All lanes : Anti-Cellubrevin antibody (ab5789)

    Lane 1 : Mouse Brain
    Lane 2 : Rat Brain
    Lane 3 : Mouse Liver
    Lane 4 : Rat Liver

    Predicted band size: 12 kDa
    Additional bands at: ~15 kDa. We are unsure as to the identity of these extra bands.



    Relative expression of Cellubrevin observed in Mouse Brain and Rat Brain in comparison to Mouse Liver and Rat Liver using ab5789 to demonstrate specificty of expression of target across different tissues. 

  • Western blot - Anti-Cellubrevin antibody (ab5789)
    Western blot - Anti-Cellubrevin antibody (ab5789)

    Western blot analysis of recombinant rat VAMP-3 using ab5789.

  • Western blot - Anti-Cellubrevin antibody (ab5789)
    Western blot - Anti-Cellubrevin antibody (ab5789)

    Western blot analysis of VAMP-3 in HeLa cell extract using ab5789.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody (ab5789)
    Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody (ab5789)

    Immunofluorescent analysis of formalin-fixed, 0.1% Triton™ X-100 permeabilized, NIH/3T3 (Mouse embryonic fibroblast cell line) cells, blocked with 3% BSA-PBS for 30 minutes at room temperature. Labelling cellubrevin with ab5789 at 1/50 dilution and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody at room temperature in the dark (right image). Compared with negative control without primary antibody (left image). Nuclei were stained with DAPI (Blue). Actin was stained with Alexa Fluor® 555 (Red). The images were captured at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody (ab5789)
    Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody (ab5789)

    Immunofluorescent analysis of formalin-fixed, 0.1% Triton™ X-100 permeabilized, Neuro-2a (Mouse neuroblastoma cell line) cells, blocked with 3% BSA-PBS for 30 minutes at room temperature. Labelling cellubrevin with ab5789 at 1/50 dilution and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody at room temperature in the dark (right image). Compared with negative control without primary antibody (left image).  Nuclei were stained with DAPI (Blue).The images were captured at 60X magnification.

プロトコール

  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • Datasheet download

    Download

参考文献 (5)

ab5789 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab5789 は 5 報の論文で使用されています。

  • Lasic E  et al. Astrocyte Specific Remodeling of Plasmalemmal Cholesterol Composition by Ketamine Indicates a New Mechanism of Antidepressant Action. Sci Rep 9:10957 (2019). PubMed: 31358895
  • Habtemichael EN  et al. Usp25m protease regulates ubiquitin-like processing of TUG proteins to control GLUT4 glucose transporter translocation in adipocytes. J Biol Chem 293:10466-10486 (2018). PubMed: 29773651
  • Verboogen DRJ  et al. Secretory vesicles of immune cells contain only a limited number of interleukin 6 molecules. FEBS Lett 592:1535-1544 (2018). PubMed: 29570778
  • Verboogen DRJ  et al. Fluorescence Lifetime Imaging Microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation. Elife 6:N/A (2017). WB, ICC/IF ; Human . PubMed: 28524818
  • Caceres PS  et al. Vesicle-associated membrane protein 2 (VAMP2) but Not VAMP3 mediates cAMP-stimulated trafficking of the renal Na+-K+-2Cl- co-transporter NKCC2 in thick ascending limbs. J Biol Chem 289:23951-62 (2014). WB ; Rat . PubMed: 25008321

レビューと Q&A

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1-8 of 8 Abreviews or Q&A

Western blot abreview for Anti-Cellubrevin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (4-20 % gradient gel)
Sample
Human Cell lysate - whole cell (HeLa, U266)
Specification
HeLa, U266
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Dec 13 2013

Question

I do not know when the antibody arrived, but it was stored in the freezer until I tested it. The attachment is a power point slide, would a word document be better? A credit note would be good for the failed antibody. Best Wishes

Read More

Abcam community

Verified customer

Asked on Jan 13 2012

Answer

Thank you for your note. I am sorry to hear that even the replacement vial failed to work. This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of one vial of ab68833. For your information, the internal reference note for this credit is CN19268. You can use this credit in the near future for any of the products which are in the catalogue. I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More

Abcam Scientific Support

Answered on Jan 13 2012

Question

I do not know when the antibody arrived, but it was stored in the freezer until I tested it. The attachment is a power point slide, would a word document be better? A credit note would be good for the failed antibody. What about the mouse monoclonal that also failed? I also tested your sheep antibody ab72402 which work well, but a picture is not included. Please let me know if you want to see that picture also. Best Wishes

Read More

Abcam community

Verified customer

Asked on Jan 10 2012

Answer

Thank you for your response and for reattaching the image. I can arrange the credit note for the cost of ab68833, our Abpromise applies for this complaint. Initially, the problem was reported only with ab68833 and looking at our system there is no record for a complaint for the other antibody ab81610. Every single complaint needs to be reported and investigated independently. As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased. Regarding ab81610: - Could you please confirm the batch and order number, - Please specify the secondary antibody used for detection I look forward to hearing from you soon.

Read More

Abcam Scientific Support

Answered on Jan 10 2012

Question

Happy New Year. I have tested the second aliquot of the Cellubrevin antibody ab68833 and compared it directly to another of your Cellubrevin antibodies ab5789. Unfortunately it still doesn’t work whereas ab5789 gives good bands in all samples. I have also tested another of your Cellubrevin antibodies in the same experiment a mouse monoclonal ab81610, which again does not give any bands. Please attached slide. What happens now? Best Wishes

Read More

Abcam community

Verified customer

Asked on Jan 09 2012

Answer

Thank you for your new contact and updates. Unfortunately, one the files you have kindly sent to us can’t be opened so I am afraid I am unable to take a look at it. Could you resend it to me? According to our last correspondence (16th December 2011), you did not receive the replacement vial of this antibody which was sent to your Company on 1st December. - When you received the replacement vial finally? - How was the antibody stored? I understand that you are satisfied with another Abcam product (ab5789) which worked as it expected. I could arrange a credit note for you for the cost of the original ab68833 which you can use in future purchase. Let me know how you wish to proceed. I look forward to hearing from you soon.

Read More

Abcam Scientific Support

Answered on Jan 09 2012

Question

we have purchased the rabbit polyclonal antibody to VAMP3 (Abcam ab5789) and used it for immunocytochemistry at the dilution 1:1000 and at our surprise we did not observed punctuate-vesicle structures only in the cytoplasm, but also a similar, but stronger, pattern in the nucleus. With the confocal microscope we also made Z-stacks, to exclude that there was only a surface labelling of the nucleus. We are questioning about the specificity of this antibody. I would be very greateful for your reply.

Read More

Abcam community

Verified customer

Asked on Mar 07 2006

Answer

Thank you for your email. Ab5789 was characterized for use in Western blotting and was not tested in other applications. By Western blot, the antibody detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa) representing recombinant rat VAMP-3. The immunogen used to generate this antibody was a synthetic peptide: STGVPSGSSAATGSNRRC, corresponding to amino acids 1-18 of Mouse VAMP3. Ab5789 has not been tested for cross-reactivity with the other VAMP isoforms. However, if you BLAST the peptide sequence used to generate this antibody (the ExPASy and NCBI links are on the online datasheet) there is no identity with other VAMPs. We do have a review from a customer who used this antibody in WB and IF (you can read her comments by clicking on the Abreviews tab located on the online product datasheet). She states that the antibody worked well for WB, but states "I expected the VAMP3 AB to have a more localized perinuclear staining, but the staining of this AB is homogeneous throughout the entire cell." It does seem that this antibody may not be suitable for IF. I do suggest that you submit an Abreview via the product datasheet. We always encourage customers to feed their results back to us, whether positive or negative, and make all information about each of our products available to researchers. If you send us an Abreview, we can offer you 50 Abcam Points (a further 100 Abpoints will be offered for an image). To find out more about our Loyalty Scheme and Abpoints, please visit our website and look at Your Account panel (left bottom section on our home page) or the Abreviews section on the product datasheet. You can submit the Abreview if you click on the Abreview section (top menu bar) on the on-line datasheet of this antibody. It is a very straightforward process. Should you need any help how to submit an Abreview, then please do not hesitate to contact us again. I hope this helps. Please contact us again if you have any additional questions.

Read More

Abcam Scientific Support

Answered on Mar 08 2006

Question

Does this antibody detect other VAMPs, or is it specific for VAMP3?

Read More

Abcam community

Verified customer

Asked on Aug 08 2005

Answer

Thank you for your enquiry. Ab5789 has not been tested for cross-reactivity with the other VAMP isoforms. However, if you BLAST the peptide sequence used to generate this antibody (the ExPASy and NCBI links are on the online datasheet) there is no identity with other VAMPs. I hope this helps, if you have any additional questions please contact us again.

Read More

Abcam Scientific Support

Answered on Aug 09 2005

Question

Ab5789 is listed as a VAMP3 antibody, and ab2102 and ab2103 are listed as Cellubrevin antibodies. Are they the same? Are the peptide sequences available for ab2102 and ab2103? Have any of these antibodies been tested for cross-reactivity with guinea pig?

Read More

Abcam community

Verified customer

Asked on Feb 22 2005

Answer

Regarding ab2102 and ab2103, unfortunately the peptide sequences are not available. If you have any additional questions, please let us know.

Read More

Abcam Scientific Support

Answered on Feb 23 2005

Question

Ab5789 is listed as a VAMP3 antibody, and ab2102 and ab2103 are listed as Cellubrevin antibodies. Are they the same? Are the peptide sequences available for ab2102 and ab2103? Have any of these antibodies been tested for cross-reactivity with guinea pig?

Read More

Abcam community

Verified customer

Asked on Feb 22 2005

Answer

Thank you for your enquiry. Cellubrevin is an alternative name for VAMP3, and so these 3 antibodies target the same protein. Ab2103 has been tested for cross-reactivity with Human and Mouse, and does not cross-react with Rat or Dog. Ab2102 cross-reacts with Rat and does not cross-react with Human or Dog. Ab5789 cross-reacts with Dog, Human, Mouse and Rat. So none have been tested yet for cross-reactivity with guinea pig, to our knowledge. I will try to obtain the peptide sequences for ab2102 and ab2103 and will let you know.

Read More

Abcam Scientific Support

Answered on Feb 22 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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