保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーConstituent: Whole serum
Our Abpromise guarantee covers the use of ab2102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250 - 1/1000.|
|ICC/IF||Use at an assay dependent dilution.|
機能SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.
配列類似性Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
細胞内局在Membrane. Cell junction > synapse > synaptosome.
- Information by UniProt
- CEB antibody
- Cellubrevin antibody
- Synaptobrevin 3 antibody
Anti-Cellubrevin antibody (ab2102) at 1/1000 dilution + Rat RBL-2H3 whole cell lysates at 10 µg
Goat anti-rabbit polyclonal HRP conjugate at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Additional bands at: 14 kDa (possible post-translational modification)
Exposure time: 5 seconds
Blot was blocked with 5% milk for 1 hour at 25°C.
This picture was kindly supplied as part of the review submitted by Jennifer Chua . The image shows staining in the perinuclear region and in the peripheral endosomal structures of RAW 264.7 cells stained with ab2102 (at a dilution of 1/50 with a secondary antibody conjugated with Alexa 568).
This product has been referenced in:
- Imai A et al. Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane. Arch Oral Biol 48:597-604 (2003). Read more (PubMed: 12828989) »
- Yamamoto M et al. Reversible suppression of glutamatergic neurotransmission of cerebellar granule cells in vivo by genetically manipulated expression of tetanus neurotoxin light chain. J Neurosci 23:6759-67 (2003). Read more (PubMed: 12890769) »